Abstract
1. General Principles Of Flow Cytometry Flow cytometry is a technique in which single cells in a fluid suspension are analyzed with respect to their intrinsic lightscattering properties and are simultaneously evaluated for one or more extrinsic properties (i.e., the presence of specific molecules) using fluorescent probes. The fluorescent probe might bind directly to the targeted molecule (e.g., propidium iodide in DNA content analysis) or a fluorescent dye might be coupled to an antibody probe, to enable detection of a specific protein. By using several different fluorochromes with substantially nonoverlapping emission spectra, the laboratorian can simultaneously evaluate the expression of multiple extrinsic cellular properties. Moreover, because the flow rate of cells within the flow cytometer is rapid, thousands of cells can be analyzed in seconds. Despite the rapidity with which data can be acquired, however, because each cell is analyzed individually, multiple intrinsic and extrinsic parameters are retained for each cell; that is, flow cytometric analysis is multiparametric. In this section, we review some general principles of flow cytometry (1–6).
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DiGiuseppe, J.A. (2006). Flow Cytometry. In: Coleman, W.B., Tsongalis, G.J. (eds) Molecular Diagnostics. Humana Press. https://doi.org/10.1385/1-59259-928-1:163
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DOI: https://doi.org/10.1385/1-59259-928-1:163
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