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Amyloid Proteins pp 211–219Cite as

Murine Cerebrovascular Cells as a Cell Culture Model for Cerebral Amyloid Angiopathy

Isolation of Smooth Muscle and Endothelial Cells From Mouse Brain

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 299))

Abstract

The use of murine cerebrovascular cells, that is, endothelial and smooth muscle cells, has not been widely employed as a cell culture model for the investigation of cellular mechanisms involved in cerebral amyloid angiopathy (CAA). Difficulties in isolation and propagation of murine cerebrovascular cells and insufficient yields for molecular and cell culture studies have deterred investigators from using mice as a source for cerebrovascular cells in culture. To date, most of the literature has described isolation of smooth muscle cells or endothelial cells from human, canine, rat, guinea pig, or other large animals.

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Author information

Authors and Affiliations

  1. Departments of Psychiatry and Pharmacology, New York University School of Medicine, New York, NY

    Efrat Levy

  2. Center for Dementia Research, Nathan S. Kline Institute for Psychiatric Research, Orangeburg, NY

    Sonia S. Jung

Authors
  1. Sonia S. Jung
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  2. Efrat Levy
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Editor information

Editors and Affiliations

  1. Departments of Psychiatry and Pathology, New York University School of Medicine, New York, NY

    Einar M. Sigurdsson

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© 2005 Humana Press Inc.

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Jung, S.S., Levy, E. (2005). Murine Cerebrovascular Cells as a Cell Culture Model for Cerebral Amyloid Angiopathy. In: Sigurdsson, E.M. (eds) Amyloid Proteins. Methods in Molecular Biology™, vol 299. Humana Press. https://doi.org/10.1385/1-59259-874-9:211

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  • DOI: https://doi.org/10.1385/1-59259-874-9:211

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-337-4

  • Online ISBN: 978-1-59259-874-8

  • eBook Packages: Springer Protocols

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