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Transgenic Plants: Methods and Protocols pp 237–253Cite as

Elimination of Marker Genes From Transgenic Plants Using MAT Vector Systems

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 286))

Summary

We have developed an efficient system (Multi-Auto-Transformation [MAT] vectors) for the removal of marker genes and to increase the regeneration frequency of transgenic crops without using antibiotic selection, reducing their possible environmental impact. The MAT vector system is designed to use the oncogenes (ipt, iaaM/H, rol) of Agrobacterium, which control the endogenous levels of plant hormones and the cell responses to plant growth regulators, to differentiate transgenic cells, and to select marker-free transgenic plants. The oncogenes are combined with the site-specific recombination system (R/RS). At transformation, the oncogenes regenerate transgenic plants and then are removed by the R/RS system to generate marker-free transgenic plants. The choice of a promoter for the oncogenes and the recombinase (R) gene, the state of plant materials and the tissue culture conditions greatly affect efficiency of both the regeneration of transgenic plants and the generation of marker-free plants. We have evaluated these conditions in several plant species to increase their generation efficiency. This chapter describes our transformation protocols using MAT vectors.

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Author information

Authors and Affiliations

  1. Pulp and Paper Research Laboratory, Nippon Paper Industries Co., Ltd., Tokyo, Japan

    Hiroyasu Ebinuma, Koichi Sugita, Saori Endo, Etsuko Matsunaga & Keiko Yamada

Authors
  1. Hiroyasu Ebinuma
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  2. Koichi Sugita
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  3. Saori Endo
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  4. Etsuko Matsunaga
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  5. Keiko Yamada
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Editor information

Editors and Affiliations

  1. Instituto Valenciano de Investigaciones Agrarias, Valencia, Spain

    Leandro Peña

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© 2005 Humana Press Inc.

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Ebinuma, H., Sugita, K., Endo, S., Matsunaga, E., Yamada, K. (2005). Elimination of Marker Genes From Transgenic Plants Using MAT Vector Systems. In: Peña, L. (eds) Transgenic Plants: Methods and Protocols. Methods in Molecular Biology™, vol 286. Humana Press. https://doi.org/10.1385/1-59259-827-7:237

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  • DOI: https://doi.org/10.1385/1-59259-827-7:237

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-263-6

  • Online ISBN: 978-1-59259-827-4

  • eBook Packages: Springer Protocols

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