Abstract
Since the early 1990s, combinatorial deoxyribonucleic acid and ribonucleic acid libraries have been used to isolate specific ligands for a variety of target molecules, as well as nucleic acid-based catalysts for different reactions. These iterative procedures are based on the fact that nucleic acids can be enzymatically amplified. In this chapter, we describe the synthesis of such combinatorial libraries, their analysis, and basic procedures of in vitro selection.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Ellington, A. D. and Szostak, J. W. (1990) In vitro selection of RNA molecules that bind specific ligands Nature 346, 818–822.
Ellington, A. D. and Szostak, J. W. (1992) Selection in vitro of single-stranded DNA molecules that fold into specific ligand-binding structures Nature 355, 850–852.
Tuerk, C. and Gold, L. (1990) Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase Science 249, 505–510.
Famulok, M. and Szostak, J. W. (1992) In vitro-Selektion spezifisch ligandenbindender Nucleinsaeuren Angew. Chem. Int. Ed. Engl. 104, 1001–1011.
Gold, L., Polisky, B., Uhlenbeck, O., and Yarus, M. (1995) Diversity of oligonucleotide functions Annu. Rev. Biochem. 64,763–797.
Jächke, A., Frauendorf, C., and Hausch, F. (1999) In vitro selected oligonucleotides as tools in organic chemistry Synlett 825–833.
Joyce, G. F. (1989) Amplification, mutation and selection of catalytic RNA Gene 82, 83–87.
Bartel, D. P. and Szostak, J. W. (1993) Isolation of new ribozymes from a large pool of random sequences Science 261, 1411–1418.
Lorsch, J. R. and Szostak, J. W. (1994) In vitro evolution of new ribozymes with polynucleotide kinase activity Nature 371, 31–36.
Tarasow, T. M., Tarasow, S. L., and Eaton, B. E. (1997) RNA-catalysed carboncarbon bond formation Nature 389, 54–57.
Wiegand, T. W., Janssen, R. C., and Eaton, B. E. (1997) Selection of RNA amide synthases Chem. Biol. 4, 675–683.
Hausch, F. and Jächke, A. (1997) Libraries of multifunctional RNA conjugates for the selection of new RNA catalysts Bioconjug. Chem. 8, 885–890.
Seelig, B. and Jächke, A. (1999) A small catalytic RNA motif with Diels-Alderase activity Chem. Biol. 6, 167–176.
Seelig, B., Keiper, S., Stuhlmann, F., and Jächke, A. (2000) Enantioselective ribozyme catalysis of a bimolecular cycloaddition reaction Angew. Chem. Int. Ed. 39, 4576–4579.
Stuhlmann, F. and Jächke, A. (2002) Characterization of an RNA active site: interactions between a Diels-Alderase ribozyme and its substrates and products. J. Am. Chem. Soc. 124, 3238–3244.
Seelig, B. and Jächke, A. (1999) Ternary conjugates of guanosine monophosphate as initiator nucleotides for the enzymatic synthesis of 5′′-modified RNAs Bioconjug. Chem. 10, 371–378.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2005 Humana Press Inc.
About this protocol
Cite this protocol
Jächke, A. (2005). In Vitro Selection From Combinatorial Nucleic Acid Libraries. In: Herdewijn, P. (eds) Oligonucleotide Synthesis. Methods in Molecular Biology, vol 288. Humana Press. https://doi.org/10.1385/1-59259-823-4:379
Download citation
DOI: https://doi.org/10.1385/1-59259-823-4:379
Publisher Name: Humana Press
Print ISBN: 978-1-58829-233-9
Online ISBN: 978-1-59259-823-6
eBook Packages: Springer Protocols