Skip to main content

Incorporation and Analysis of LHCII in Model Systems

  • Protocol
Photosynthesis Research Protocols

Part of the book series: Methods In Molecular Biology™ ((MIMB,volume 274))

Abstract

The largest light-harvesting pigment-protein complex of Photosystem II (LHCII) is an antenna complex comprising more than half of photosynthetically active chlorophyll pigments. Understanding photophysical processes in this pigment-protein complex and the relationship between the structure and function of LHCII has been the subject of numerous studies, including those in the model systems, such as monomolecular layers, Langmuir-Blodgett films, planar lipid bilayers, and liposomes. This chapter describes the methods of the incorporation of LHCII into these particular model systems, control of intactness of LHCII after incorporation, and the examination of this pigment-protein complex in model systems.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 74.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Gaines, G. L. (1966) Insoluble Monolayers at Liquid-Gas Interfaces. Wiley, New York.

    Google Scholar 

  2. Munger, G., Lorrain, L., Gagne, G., and Leblanc, R. M. (1987) Adjustable sped and vibration-free hydraulic system for Langmuir-Blodgett films deposition. Rev. Sci. Instrum. 58, 285–288.

    Article  CAS  Google Scholar 

  3. Gruszecki, W. I., Zelent, B., Tajmir-Riahi, et al. (2000) Chlorophyll a-violaxanthin interactions in monolayers at air-water interface and Langmuir-Blodgett films. Colloids Surfaces B: Biointerfaces 19, 117–125.

    Article  CAS  Google Scholar 

  4. Niedzwiedzki, D. and Gruszecki, W. I. (2003) Interaction between chlorophyll a and violaxanthin in different steric conformations. Model studies in monomolecular layers. Colloids Surfaces B: Biointerfaces 28, 27–38.

    Article  CAS  Google Scholar 

  5. Gruszecki, W. I., Grudzinski, W., Matula, M., Kernen, P., and Krupa, Z. (1999) Light-induced excitation quenching and structural transition in light-harvesting complex II. Photosynth. Res. 59, 175–185.

    Article  CAS  Google Scholar 

  6. Kernen, P., Gruszecki, W. I., Matula, M., Wagner, P., Ziegler, U., and Krupa, Z. (1998) Light-harvesting complex II in monocomponent and mixed lipid-protein monolayers. Biochim. Biophys. Acta 1373, 289–298.

    Article  PubMed  CAS  Google Scholar 

  7. Grudzinski, W., Krupa, Z., Garstka, M., Maksymiec, W., Swartz, T. E., and Gruszecki, W. I. (2002) Conformational rearrangements in Light-Harvesting Complex II accompanying light-induced chlorophyll a fluorescence quenching. Biochim. Biophys. Acta 1554, 108–117.

    Article  PubMed  CAS  Google Scholar 

  8. Gruszecki, W. I., Kernen, P., Krupa, Z., and Strasser, R. J. (1994) Involvement of xanthophyll pigments in regulation of light-driven excitation quenching in light-harvesting complex of Photosystem II. Biochim. Biophys. Acta 1188, 235–242.

    Article  Google Scholar 

  9. Wardak, A., Brodowski, R., Krupa, Z., and Gruszecki, W. I. (2000) Effect of light-harvesting complex II on ion transport across model lipid membranes. J. Photochem. Photobiol. B: Biol. 56, 12–18.

    Article  CAS  Google Scholar 

  10. Gruszecki, W. I., Wardak, A., and Maksymiec, W. (1997) The effect of blue light on electron transport in photosystem II reconstituted in planar bilayer lipid membrane. J. Photochem. Photobiol. B: Biol. 39, 265–268.

    Article  CAS  Google Scholar 

  11. Grudzinski, W., Matula, M., Sielewiesiuk, J., Kernen, P., Krupa, Z., and Gruszecki, W. I. (2001) Effect of 13-cis violaxanthin on organization of light harvesting complex II in monomolecular layers. Biochim. Biophys. Acta 1503, 291–302.

    Article  PubMed  CAS  Google Scholar 

  12. Kühlbrandt, W., Wang, D. N., and Fujiyoshi, Y. (1994) Atomic model of plant light-harvesting complex by electron crystallography. Nature 367, 614–621.

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2004 Humana Press Inc., Totowa, NJ

About this protocol

Cite this protocol

Gruszecki, W.I. (2004). Incorporation and Analysis of LHCII in Model Systems. In: Carpentier, R. (eds) Photosynthesis Research Protocols. Methods In Molecular Biology™, vol 274. Humana Press. https://doi.org/10.1385/1-59259-799-8:173

Download citation

  • DOI: https://doi.org/10.1385/1-59259-799-8:173

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-232-2

  • Online ISBN: 978-1-59259-799-4

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics