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Photosynthesis Research Protocols pp 115–128Cite as

Separation, Purification, and Characterization of Polypeptide Composition of Subcomplexes of the Main Light-Harvesting Chlorophyll a/b-Protein Complex of Photosystem II

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Part of the book series: Methods In Molecular Biology™ ((MIMB,volume 274))

Abstract

The protocols regarding separation and purification of LHCII subcomplexes by non-denaturing isoelectric focusing (ndIEF), confirmation of trimeric nature of the subcomplexes, and establishing their polypeptide composition are described below.

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Author information

Authors and Affiliations

  1. Department of Plant Physiology, Adam Mickiewicz University, Poznan, Poland

    Grzegorz Jackowski

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  1. Grzegorz Jackowski
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Editors and Affiliations

  1. Départment de Chimie-Biologie (GREIB), Université du Québec à Trois-Rivières, Trois-Rivières, Québec, Canada

    Robert Carpentier

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© 2004 Humana Press Inc., Totowa, NJ

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Jackowski, G. (2004). Separation, Purification, and Characterization of Polypeptide Composition of Subcomplexes of the Main Light-Harvesting Chlorophyll a/b-Protein Complex of Photosystem II. In: Carpentier, R. (eds) Photosynthesis Research Protocols. Methods In Molecular Biology™, vol 274. Humana Press. https://doi.org/10.1385/1-59259-799-8:115

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  • DOI: https://doi.org/10.1385/1-59259-799-8:115

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-232-2

  • Online ISBN: 978-1-59259-799-4

  • eBook Packages: Springer Protocols

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