Abstract
Procedures to isolate native light-harvesting chlorophyll-a/b complex (LHCIIb) and to reconstitute recombinant LHCIIb are described. Separation of trimeric from monomeric forms and free pigment by sucrose density-gradient ultracentrifugation can be applied to both native and reconstituted complexes. The preparations are characterized by their pigment composition, protein pattern and spectral properties.
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© 2004 Humana Press Inc., Totowa, NJ
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Rühle, W., Paulsen, H. (2004). Preparation of Native and Recombinant Light-Harvesting Chlorophyll-a/b Complex. In: Carpentier, R. (eds) Photosynthesis Research Protocols. Methods In Molecular Biology™, vol 274. Humana Press. https://doi.org/10.1385/1-59259-799-8:093
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DOI: https://doi.org/10.1385/1-59259-799-8:093
Publisher Name: Humana Press
Print ISBN: 978-1-58829-232-2
Online ISBN: 978-1-59259-799-4
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