Abstract
Methods to prepare photosystem (PS)I reaction center (RC) subunit polypeptides are described. For PsaC, PsaD, PsaE, and PsaY, the filtrate resulting from the ultrafiltration in Chapter 6 is used for preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). PsaA and PsaB, Type II PSI particles, prepared as described in the preceding chapter, are treated with sodium dodecyl sulfate, then applied on preparative SDS-PAGE. After electrophoresis, each band is cut out and electroeluted. A simple method to prepare PsaC is also presented.
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References
Hiyama, T., Yumoto, K., Satoh, A., et al. (2000) Chromatographic separation of a small subunit (PsbW/PsaY) and its assignment to Photosystem I RC. Biochim. Biophys. Acta 1459, 117–124.
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© 2004 Humana Press Inc., Totowa, NJ
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Hiyama, T. (2004). Isolation of Photosystem I Reaction Center Subunit Polypeptides From Spinach. In: Carpentier, R. (eds) Photosynthesis Research Protocols. Methods In Molecular Biology™, vol 274. Humana Press. https://doi.org/10.1385/1-59259-799-8:063
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DOI: https://doi.org/10.1385/1-59259-799-8:063
Publisher Name: Humana Press
Print ISBN: 978-1-58829-232-2
Online ISBN: 978-1-59259-799-4
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