Abstract
Methods to isolate and purify 6- and 5-chlorophyll (Chl) D1/D2/Cyt b 559 photosystem (PS) II reaction center complexes from plants are presented, and the advantages and disadvantages of each procedure are discussed. One of the simpler 6-Chl procedures and a procedure for isolating 5-Chl complexes are described in detail. Furthermore, a rapid procedure that produces relatively large amounts of less pure 6-Chl material is also described. Criteria to assess the purity of photosystem II (PSII) reaction center preparations are presented, and problems associated with each of the isolation procedures are discussed.
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References
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© 2004 Humana Press Inc., Totowa, NJ
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Seibert, M., Yruela, I., Picorel, R. (2004). Isolation of Photosystem II Reaction Center Complexes From Plants. In: Carpentier, R. (eds) Photosynthesis Research Protocols. Methods In Molecular Biology™, vol 274. Humana Press. https://doi.org/10.1385/1-59259-799-8:053
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DOI: https://doi.org/10.1385/1-59259-799-8:053
Publisher Name: Humana Press
Print ISBN: 978-1-58829-232-2
Online ISBN: 978-1-59259-799-4
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