Abstract
Methods for the isolation of highly active oxygen-evolving photosystem (PS)II membranes from higher plants and the purification of the oxygen-evolving complex (OEC) subunits are described. Membrane samples used as the material for various in vitro studies of PSII are prepared by solubilization of thylakoid membranes with the non-ionic detergent Triton X-100. The OEC subunit proteins are dissociated from the PSII-enriched membranes by alkaline treatment or salt treatment, and then purified by ion-exchange chromatography using an automated high-performance liquid chromatography system.
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References
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© 2004 Humana Press Inc., Totowa, NJ
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Yamamoto, Y., Sakuma, S., Shen, JR. (2004). Isolation of Photosystem II-Enriched Membranes and the Oxygen-Evolving Complex Subunit Proteins From Higher Plants. In: Carpentier, R. (eds) Photosynthesis Research Protocols. Methods In Molecular Biology™, vol 274. Humana Press. https://doi.org/10.1385/1-59259-799-8:029
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DOI: https://doi.org/10.1385/1-59259-799-8:029
Publisher Name: Humana Press
Print ISBN: 978-1-58829-232-2
Online ISBN: 978-1-59259-799-4
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