Abstract
A method to prepare photosystem I (PSI) particles is described. Spinach leaves are used to prepare broken chloroplasts that are then solubilized by using a detergent (Triton X-100). Solubilized chloroplasts are then applied on an ion-exchange column. Eluted by a linear concentration gradient of NaCl, fractions enriched in PSI particles are collected and applied on a small hydroxyapatite column. By eluting with phosphate buffer, a concentrated preparation of PSI particles is obtained. The particles consist of PsaA, PsaB, PsaC, PsaD, PsaE, and PsaY. Assay methods that involve SDS-PAGE and P700 determination are also presented.
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References
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© 2004 Humana Press Inc., Totowa, NJ
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Hiyama, T. (2004). Isolation of Photosystem I Particles From Spinach. In: Carpentier, R. (eds) Photosynthesis Research Protocols. Methods In Molecular Biology™, vol 274. Humana Press. https://doi.org/10.1385/1-59259-799-8:011
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DOI: https://doi.org/10.1385/1-59259-799-8:011
Publisher Name: Humana Press
Print ISBN: 978-1-58829-232-2
Online ISBN: 978-1-59259-799-4
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