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Stable Gene Delivery to CNS Cells Using Lentiviral Vectors

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Gene Delivery to Mammalian Cells

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 246))

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Abstract

Recombinant viral vectors have been used to study a variety of fundamental issues in developmental neurobiology, as well as pathogenesis and treatments for various neurodegenerative diseases. Lentiviral vectors are valuable tools for neurobiology research owing to their ability to transduce nondividing cells, such as neurons, and to introduce therapeutic or reporter genes into central nervous system (CNS) cells in vivo and in vitro.

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Author information

Authors and Affiliations

  1. Department of Pathobiology and Center for Comparative Molecular Genetics, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA

    Deborah J. Watson

  2. Department of Neurology, The Children’s Hospital of Philadelphia, Philadelphia, PA

    Deborah J. Watson

  3. Department of Pathobiology and Center for Comparative Medical Genetics, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA

    Brian A. Karolewski & John H. Wolfe

  4. Division of Neurology, Children’s Hospital of Philadelphia, Philadelphia, PA

    Brian A. Karolewski & John H. Wolfe

Authors
  1. Deborah J. Watson
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  2. Brian A. Karolewski
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  3. John H. Wolfe
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Editor information

Editors and Affiliations

  1. Bio-Rad Laboratories, Hercules, CA

    William C. Heiser

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© 2004 Humana Press Inc., Totowa, NJ

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Watson, D.J., Karolewski, B.A., Wolfe, J.H. (2004). Stable Gene Delivery to CNS Cells Using Lentiviral Vectors. In: Heiser, W.C. (eds) Gene Delivery to Mammalian Cells. Methods in Molecular Biology™, vol 246. Humana Press. https://doi.org/10.1385/1-59259-650-9:413

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  • DOI: https://doi.org/10.1385/1-59259-650-9:413

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-095-3

  • Online ISBN: 978-1-59259-650-8

  • eBook Packages: Springer Protocols

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