Abstract
Adenoviruses, which were first isolated in the 1950s, have been developed as gene-delivery vehicles, or vectors, since the early 1980s (1). The adenoviruses constitute the Adenoviridae family, which is divided into two genera: the Aviadenovirus genus infects only birds, whereas the Mastadenovirus genus contains viruses that infect a range of mammalian species. Human adenoviruses are classified into six subgroups based on the percentage of guanine and cytosine in the DNA molecules and the ability to agglutinate red blood cells. They are further subdivided into more than 50 serotypes, primarily on the basis of neutralization assays (reviewed in ref. 2).
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References
Berkner, K. L. (1988) Development of adenovirus vectors for the expression of heterologous genes. Biotechniques 6, 616–629.
Shenk, T. (1996) Adenoviridae: The viruses and their replication, in Fields Virology, (Fields, B. N., Knipe, D. M., Howley, P. M., Chanock, R. M., Melnick, J. L., Monath, T. P., et al., eds.), Lippincott-Raven Publishers, Philadelphia, PA, pp. 2111–2148.
Kovesdi, I., Brough, D. E., Bruder, J. T., and Wickham, T. J. (1997) Adenoviral vectors for gene transfer. Curr. Opin. Biotechnol. 8, 583–589.
Curiel, D. T. and Douglas, J. T. (2002) Adenoviral Vectors for Gene Therapy. Academic Press, New York, NY.
Bergelson, J. M., Cunningham, J. A., Droguett, G., Kurt-Jones, E. A., Krithivas, A., Hong, J. S., et al. (1997) Isolation of a common receptor for coxsackie B viruses and adenoviruses 2 and 5. Science 275, 1320–1323.
Tomko, R. P., Xu, R., and Philipson, L. (1997) HCAR and MCAR: the human and mouse cellular receptors for subgroup C adenoviruses and group B coxsackie-viruses. Proc. Natl. Acad. Sci. USA 94, 3352–3356.
Henry, L. J., Xia, D., Wilke, M. E., Deisenhofer, J., and Gerard, R. D. (1994) Characterization of the knob domain of the adenovirus type 5 fiber protein expressed in Escherichia coli. J. Virol. 68, 5239–5246.
Louis, N., Fender, P., Barge, A., Kitts, P., and Chroboczek, J. (1994) Cell-binding domain of adenovirus serotype 2 fiber. J. Virol. 68, 4104–4106.
Leon, R. P., Hedlund, T., Meech, S. J., Li, S., Schaack, J., Hunger, S. P., Duke, R. C., and DeGregori, J. (1998) Adenoviral-mediated gene transfer in lymphocytes. Proc. Natl. Acad. Sci. USA 95, 13159–13164.
Wang, X. and Bergelson, J. M. (1999) Coxsackievirus and adenovirus receptor cytoplasmic and transmembrane domains are not essential for coxsackievirus and adenovirus infection. J. Virol. 73, 2559–2562.
Bai, M., Harfe, B., and Freimuth, P. (1993) Mutations that alter an Arg-Gly-Asp (RGD) sequence in the adenovirus type 2 penton base protein abolish its cell-rounding activity and delay virus reproduction in flat cells. J. Virol. 67, 5198–5205.
Wickham, T. J., Mathias, P., Cheresh, D. A., and Nemerow, G. R. (1993) Integrins alpha v beta 3 and alpha v beta 5 promote adenovirus internalization but not virus attachment. Cell 73, 309–319.
Danthinne, X. and Imperiale, M. J. (2000) Production of first generation adenovirus vectors: a review. Gene Ther. 7, 1707–1714.
Chinnadurai, G., Chinnadurai, S., and Brusca, J. (1979) Physical mapping of a large-plaque mutation of adenovirus type 2. J. Virol. 32, 623–628.
McGrory, W. J., Bautista, D. S., and Graham, F. L. (1988) A simple technique for the rescue of early region I mutations into infectious human adenovirus type 5. Virology 163, 614–617.
Bett, A. J., Haddara, W., Prevec, L., and Graham, F. L. (1994) An efficient and flexible system for construction of adenovirus vectors with insertions or deletions in early regions 1 and 3. Proc. Natl. Acad. Sci. USA 91, 8802–8806.
Chartier, C., Degryse, E., Gantzer, M., Dieterle, A., Pavirani, A., and Mehtali, M. (1996) Efficient generation of recombinant adenovirus vectors by homologous recombination in Escherichia coli. J. Virol. 70, 4805–4810.
Crouzet, J., Naudin, L., Orsini, C., Vigne, E., Ferrero, L., Le Roux, A., et al. (1997) Recombinational construction in Escherichia coli of infectious adenoviral genomes. Proc. Natl. Acad. Sci. USA 94, 1414–1419.
He, T. C., Zhou, S., da Costa, L. T., Yu, J., Kinzler, K. W., and Vogelstein, B. (1998) A simplified system for generating recombinant adenoviruses. Proc. Natl. Acad. Sci. USA 95, 2509–2514.
Graham, F. L., Smiley, J., Russell, W. C., and Nairn, R. (1977) Characteristics of a human cell line transformed by DNA from human adenovirus type 5. J. Gen. Virol. 36, 59–74.
Lochmuller, H., Jani, A., Huard, J., Prescott, S., Simoneau, M., Massie, B., Karpati, G, and Acsadi, G (1994) Emergence of early region 1-containing replication-competent adenovirus in stocks of replication-defective adenovirus recombinants (delta E1 + delta E3) during multiple passages in 293 cells. Hum. Gene Ther. 5, 1485–1491.
Fallaux, F J., Bout, A., van der Velde, I., van den Wollenberg, D. J., Hehir, K. M., Keegan, J., et al. (1998) New helper cells and matched early region 1-deleted adenovirus vectors prevent generation of replication-competent adenoviruses. Hum. Gene Ther. 9, 1909–1917.
Gao, G. P., Engdahl, R. K., and Wilson, J. M. (2000) A cell line for high-yield production of E1-deleted adenovirus vectors without the emergence of replication-competent virus. Hum. Gene Ther. 11, 213–219.
Shabram, P., Vellekamp, G., and Scandella, C. (2002) Purification of adenovirus, in Adenoviral Vectors for Gene Therapy (Curiel, D. T. and Douglas, J. T., eds.), Academic Press, New York, NY, pp. 167–204.
Mittereder, N., March, K. L., and Trapnell, B. C. (1996) Evaluation of the concentration and bioactivity of adenovirus vectors for gene therapy. J. Virol. 70, 7498–7509.
Maizel, J. V., Jr., White, D. O., and Scharff, M. D. (1968) The polypeptides of adenovirus. I. Evidence for multiple protein components in the virion and a comparison of types 2, 7A, and 12. Virology 36, 115–125.
Brenner, M. (1999) Gene transfer by adenovectors. Blood 94, 3965–3967.
Young, L. S. and Mautner, V. (2001) The promise and potential hazards of adenovirus gene therapy. Gut 48, 733–736.
Barnett, B. G., Crews, C. J., and Douglas, J. T (2002) Targeted adenoviral vectors. Biochim. Biophys. Acta 1575, 1–14.
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Douglas, J.T. (2004). Adenovirus-Mediated Gene Delivery. In: Heiser, W.C. (eds) Gene Delivery to Mammalian Cells. Methods in Molecular Biology™, vol 246. Humana Press. https://doi.org/10.1385/1-59259-650-9:3
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DOI: https://doi.org/10.1385/1-59259-650-9:3
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