Abstract
The identification of proteins using preparative gel electrophoresis and mass spectrometry requires reversible staining of relatively thick (1–1.5 mm) polyacrylamide gels. We have found that staining with colloidal Coomassie brilliant blue G-250 or negative staining with imidazole-zinc yields high-resolution stains (Fig. 1) that are compatible with subsequent mass spectrometric analysis (see Notes 1 and 5).
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References
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© 1999 Humana Press Inc., Totowa, NJ
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Matsui, N.M., Smith-Beckerman, D.M., Epstein, L.B. (1999). Staining of Preparative 2-D Gels. In: Link, A.J. (eds) 2-D Proteome Analysis Protocols. Methods in Molecular Biology, vol 112. Humana Press. https://doi.org/10.1385/1-59259-584-7:307
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DOI: https://doi.org/10.1385/1-59259-584-7:307
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