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PCR Protocols pp 271–283Cite as

Serial Analysis of Gene Expression

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 226))

Abstract

Serial analysis of gene expression (SAGE™) is a patented, large-scale mRNA-profiling technology that produces comprehensive, quantitative, and reproducible gene expression profiles (originally described in refs. 1 and 2). Unlike the alternative technologies of differential display and subtractive hybridization, SAGE produces a full catalog of all transcripts, not only differentially expressed genes, and unlike smaller arrays, SAGE needs no assumptions about the genes that are likely to be expressed, thus allowing the identification of novel genes (are among many excellent reviews, see refs. 3–5). SAGE is based on generating clones of concatenated (linked) short sequence tags derived from mRNA from the target cells or tissue (Fig. 1).

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Author information

Authors and Affiliations

  1. CRC Department of Medical Oncology, University of Glasgow, Glasgow, UK

    Karin A. Oien

Authors
  1. Karin A. Oien
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Editor information

Editors and Affiliations

  1. Department of Surgery, Glasgow Royal Infirmary, Glasgow, UK

    John M. S. Bartlett

  2. Department of Hematology, Royal Infirmary of Edinburgh, Edinburgh, UK

    David Stirling

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© 2003 Humana Press Inc.

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Oien, K.A. (2003). Serial Analysis of Gene Expression. In: Bartlett, J.M.S., Stirling, D. (eds) PCR Protocols. Methods in Molecular Biology™, vol 226. Humana Press. https://doi.org/10.1385/1-59259-384-4:271

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  • DOI: https://doi.org/10.1385/1-59259-384-4:271

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-642-0

  • Online ISBN: 978-1-59259-384-2

  • eBook Packages: Springer Protocols

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