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Bicistronic GFP Expression Vectors as a Tool to Study Ion Channels in Transiently Transfected Cultured Cells

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Ion Channel Localization

Part of the book series: Methods in Pharmacology and Toxicology ((MIPT))

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Abstract

The availability of ion-channel cDNAs has greatly increased our insight in the structure, function, pharmacology, and regulation of ion channels at the molecular level. Much of this knowledge has been obtained by expressing wild-type or mutant ion channels in a heterologous host system, thereby facilitating functional approaches and analyses, which are not possible, when the native channel is studied in its in situ context (1).

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Author information

Authors and Affiliations

  1. Laboratory of Physiology, Catholic University of Leuven, Leuven, Belgium

    Jan Eggermont, Dominique Trouet, Gunnar Buyse, Rudi Vennekens, Guy Droogmans & Bernd Nilius

Authors
  1. Jan Eggermont
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  2. Dominique Trouet
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  3. Gunnar Buyse
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  4. Rudi Vennekens
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  5. Guy Droogmans
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  6. Bernd Nilius
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Editor information

Editors and Affiliations

  1. University of Michigan, Ann Arbor, MI

    Anatoli N. Lopatin

  2. Washington University Medical School, St. Louis, MO

    Colin G. Nichols

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© 2001 Humana Press Inc., Totowa, NJ

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Eggermont, J., Trouet, D., Buyse, G., Vennekens, R., Droogmans, G., Nilius, B. (2001). Bicistronic GFP Expression Vectors as a Tool to Study Ion Channels in Transiently Transfected Cultured Cells. In: Lopatin, A.N., Nichols, C.G. (eds) Ion Channel Localization. Methods in Pharmacology and Toxicology. Humana Press. https://doi.org/10.1385/1-59259-118-3:167

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  • DOI: https://doi.org/10.1385/1-59259-118-3:167

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-833-2

  • Online ISBN: 978-1-59259-118-3

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