Abstract
Biological effects of steroids are mediated through an initial interaction with specific receptor belonging to a member of the steroid/thyroid/retinoid receptor gene superfamily (1,2). Recently, increasing numbers of new members of this family, including their subtypes have been cloned and characterized. For instance, until the recent cloning of a second estrogen receptor (ER) α, ERβ had been considered as the only receptor able to bind estrogen with high affinity. ERα protein is smaller than ERβ protein but has a similar high affinity for estradiol, as does the receptor (3,4). Both receptors demonstrate high conservation of amino acid sequence in regions of the hormone-binding domain known to be important in contacting ligands (3). However, tissue distribution and relative expression levels of ERα have been demonstrated to be different from those of ERβ, which suggests possible different biological roles of ERα in mammalian estrogen dependent tissues (5).
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References
Auchus, J. A. and Fuqua, S. A. W. (1994) The oestrogen receptor. Bailliere Clin. Endocrinol. Metab. 8, 433–449.
Tsai, M. J. and O’Malley, B. W. (1994) Molecular mechanisms of action of steroid/ thyroid receptor superfamily. Annu. Rev. Biochem. 63, 451–486.
Kuiper, G. G. J. M., Enmark, E., Puelto-Huiko, M., Nilsson., S., and Gustafsson., J. A. (1996) Cloning of a novel estrogen receptor expressed in rat prostate and ovary. Proc. Natl. Acad. Sci. USA 93, 2925–2930.
Mosselman, S., Polman, J., and Dijkema, R. (1996) ER-beta: identification and characterization of a novel human estrogen receptor. FEBS Lett. 392, 49–53.
Enmark, E., Pelto-Houikko, M., Lagercrantz, S., Lagercrants, J., Fried, G., Nordenskjold, M., and Gustafsson, J.-A. (1997) Human estrogen receptor betagene structure, chromosomal localization, and expression pattern. J. Clin. Endocrinol. Metab. 82, 4258–4265.
Sasano, H. (1994) Application of mRNA in situ hybridization to surgical pathology materials. Acta Histochem. Cytochem. 27, 567–571.
Felger, R. E., Montone, K. T., and Furthe, E. E. (1996) A rapid method for the detection of hepatitis C virus RNA by in situ hybridization. Modern Pathol. 9, 696–702.
Park, C. S., Kim, J., and Montone, K. T. (1997) Detection of Aspergillus ribosomal RNA using biotinylated oligonucleotide probes. Diagn. Mol. Pathol. 6, 255–260.
Montone, K. T., Hodinka, R. L., Salhany, K. E., Lavi, E., Rostami, A., and Tomaszewski, J. E. (1996) Identification of Epstein-Barr virus lytic activity in post-transplantation lymphoproliferative disease. Modern Pathol. 9, 621–630.
Montone, K. T. and Litzky, L. A. (1995) Rapid method for detection of Aspergillus 5S ribosomal RNA using a genus-specific oligonucleotide probe. Am. J. Clin. Pathol. 103, 48–51.
Wang. J. Y. and Montone, K. T. (1994) A rapid simple in situ hybridization method for herpes simplex virus employing a synthetic biotin-labeled oligonucleotide probe: a comparison with immunohistochemical methods for HSV detection. J. Clin. Lab. Anal. 8, 105–115.
Matsuzaki, S., Fukaya, T., Suzuki, T., Murakami, T., Sasano H., and Yajima, A. (1999) Oestrogen receptor alpha and beta mRNA expression in human endometrium throughout the menstrual cycle. Mol. Hum. Reproduction 5, 559–564.
Iino, K., Sasano, H., Oki, Y., Andoh, N., Shin, R. W., Kitamoto, T., et al. (1999) Urocortin expression in the human central nervous system. Clin. Endocrinol. 50, 107–114.
Sasano, H., Suzuki, T., Matsuzaki, Y., Fukaya, T., Endoh, M., Nagura, H., and Kimura, M. (1999) Messenger ribonucleic acid in situ hybridization analysis of estrogen receptors alpha and beta in human breast carcinoma. J. Clin. Endocrinol. Metab. 84, 781–785.
Kim, D. H., Inagaki, Y., Suzuki, T., Ioka, R. X., Yoshioka, S. Z., Magoori, K., et al. (1998) A new low density lipoprotein receptor related protein, LRP5, is expressed in hepatocytes and adrenal cortex, and recognizes apolipoprotein E. J. Biochem. 124, 1072–1076.
Sasano, H., Uzuki, M., Sawai, T., Nagura, H., Matsunaga, G., Kashimoto, O., and Harada N. (1997) Aromatase in human bone tissue. J. Bone Miner. Res. 12, 1416–1423.
Iino, K., Sasano, H., Oki, Y., Andoh, N., Shin, R. W. et al. (1997) Urocortin expression in human pituitary gland and pituitary adenoma. J. Clin. Endocrinol. 82, 3842–3850.
Ungar, E. R. and Lee, D. R. (1995) In situ hybridization: principles and diagnostic applicatoin in infection. J. Histotechnol. 18, 203–209.
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Sasano, H., Matsuzaki, S., Suzuki, T. (2001). Estrogen Receptor mRNA In Situ Hybridization Using Microprobe System. In: Lieberman, B.A. (eds) Steroid Receptor Methods. Methods in Molecular Biology™, vol 176. Humana Press. https://doi.org/10.1385/1-59259-115-9:317
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DOI: https://doi.org/10.1385/1-59259-115-9:317
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