Abstract
Of the many methods and techniques for in vivo gene transfer, some have already been used in clinical trials. In most cases, genes are transferred into tissues using the infectivity of viral particles. However, viral systems have some known drawbacks (1,2). If an efficient and specific transfer method could be developed, naked plasmid DNA would be an ideal system for gene transfer. Plasmid-mediated methods would be economical and easy. Also, the transfer procedure could be easily repeated, as naked plasmid DNA has little antigenicity for the host (3,4).
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References
Kozarsky, K. F. and Wilson, J. M. (1993) Gene therapy: Adenovirus vectors. Curr. Opin. Genet. Dev. 3, 499–503.
Suhr, S. T. and Gage, F. H. (1993) Gene therapy for neurologic disease. Arch. Neurol. 50, 1252–1268.
Gilkeson, G. S., Pritchard, A. J., and Pisetsky, D. S. (1991) Specificity of anti-DNA antibodies induced in normal mice by immunization with bacterial DNA. Clin. Immunol. Immunopathol. 59, 288–300.
Jiao, S., Williams, P., Berg, R. K., Hodgeman, B. A., Liu, L., Repetto, G., and Wolff, J. A. (1992) Direct gene transfer into nonhuman primate myofibers in vivo. Hum. Gene Ther. 3, 21–33.
Matthews, K. E., Dev, S. B., Toneguzzo, F., and Keating, A. (1995) Electroporation for gene therapy. Methods Mol. Biol. 48, 273–280.
Mir, L. M., Banoun, H., and Paoletti, C. (1988) Introduction of definite amounts of nonpermanent molecules into living cells after electropermeabilization: Direct access to the cytosol. Exp. Cell Res. 175, 15–25.
Poddevin, B., Orlowski, S., Belehradek, J. J., and Mir, L. M. (1991) Very high cytotoxicity of bleomycin introduced into the cytosol of cells in culture. Biochem. Pharmacol. 42, 567–575.
Dev, S. B. and Hofmann, G. A. (1995) Clinical applications of electroporation, in The Electrical Manupilation of Cells (Lynch, P. T. and Davey, M. R., eds.), Chapman and Hall, New York, pp. 185–199.
Belehradek, M., Domenge, C., Luboinski, B., Orlowski, S., Belehradek, J. Jr., and Mir, L. M. (1993) Electrochemotherapy, a new antitumor treatment. First clinical phase I-II trial. Cancer 72, 3694–3700.
Nishi, T., Yoshizato, K., Yamashiro, S., Takeshima, H., Sato, K., Hamada, K., Kitamura, I., Yoshimura, T., Saya, H., Kuratsu J., and Ushio, Y. (1996) Highefficiency in vivo gene transfer using intra-arterial plasmid DNA injection following in vivo electroporation. Cancer Res. 56, 1050–1055.
Oshima, Y., Sakamoto, T., Yamanaka, I., Nishi, T., Ishibashi, T., and Inomata, H. (1998) Target gene transfer to corneal endothelium in vivo by electric pulse. Gene Ther. 5, 1347–1354.
Yoshimura, T., Yuhki, N., and Moore, S. K. (1989) Human monocyte chemoattractant protein-1 (MCP-1). Full-length cDNA cloning, expression in mitogen-stimulated blood mononuclear leukocytes, and sequence similarity to mouse competence gene JE. FEBS Lett. 244, 487–493.
Leonard, E. J. and Yoshimura, T. (1990.) Human monocyte chemoattractant protein-1 (MCP-1). Immunol. Today 11, 97–101.
Salford, L. G., Persson, B. R. R., Brun, A., Ceberg, C. P., Kongstad, P. C., and Mir, L. M. (1993) A new brain tumour therapy combining bleomycin with in vivo electropermeabilization. Biochem. Biophys. Res. Commun. 194, 938–943.
Yamaguchi, O., Irisawa, C., Baba, K., Ogihara, M., Yokota, T., and Shiraiwa, Y. (1994) Potentiation of antitumor effect of bleomycin by local electric pulses in mouse bladder tumor. Tohoku J. Exp. Med. 172, 291–293.
Belehradek, J. J., Orlowski, S., Poddevin, B., Paoletti, C., and Mir, L. M. (1991) Electrochemotherapy of spontaneous mammary tumours in mice. Eur. J. Cancer 27, 73–76.
Bondi, A., Chieregatti, G., and Eusebi, V. (1982) The use of fÀ-galactosidase as a tracer in immunocytochemistry. Histochemistry 76, 153–158.
Isobe, S., Nakane, P., and Brown, W. R. (1977) Studies on translocation of immunoglobulin across intestinal epithelium. 1. Improvement in the peroxidase-labeled antibody method for application to study of human intestinal mucosa. Acta Histochem. Cytochem. 10, 167–171.
Yoshimura, T., Takeya, M., Takahashi, K., Kuratsu, J., and Leonard, E. J. (1991) Production and characterization of mouse monoclonal antibodies against human monocyte chemoattractant protein-1. J. Immunol. 147, 2229–2233.
Herbomel, P., Bourachot, B., and Yaniv, M. (1984) Two distinct enhancers with different cell specificities coexist in the regulatory region of polyoma. Cell 39, 653–662.
Presse, F., Quillet, A., Mir, L., Marchiol-Fournigault, C., Feunteun, J., and Fradelizi, D. (1988) An improved electrotransfection method using square shaped electric impulsions. Biochem. Biophys. Res. Comm. 151, 982–990.
Takeshima, H., Kuratsu, J., Takeya, M., Yoshimura, T., and Ushio, Y. (1994) Expression and localization of messenger RNA and protein for monocyte chemoattractant protein-1 in human malignant glioma. J. Neurosurg. 80, 1056–1062.
Sato, K., Kuratsu, J., Takeshima, H., Yoshimura, T., and Ushio, Y. (1995) Expression of moncyte chemoattractant protein-1 in meningioma. J. Neurosurg. 82, 874–878.
Yamashiro, S., Takeya, M., Nishi, T., Kuratsu, J., Yoshimura, T., Ushio, Y., and Takahashi, K. (1994) Tumor-derived monocyte chemoattractant protein-1 induces intratumoral infiltration of monocyte-derived macrophage subpopulation in transplanted rat tumors. Am. J. Pathol. 55, 854–867.
Tekle, E., Astumian, R. D., and Chock, P. B. (1994) Selective and asymmetric molecular transport across electroporated cell membranes. Proc. Natl. Acad. Sci. USA 91, 11,512.11,516.
Heller, R., Jaroszeski, M., Atkin, A., Moradpour, D., Gilbert, R., Wands, J., and Nicolau, C. (1996) In vivo gene electroinjection and expression in rat liver. FEBS Lett. 389, 225–228.
Sukharev, S. I., Klenchin, V. A., Serov, S. M., Chernommordik, L. V., and Chizmadzhev, Y. A. (1992) Electroporation and electrophoretic DNA transfer into cells: The effect of DNA interaction with electropores. Biophys. J. 63, 1320–1327.
Hui, W. H. (1995) Effects of pulse length and strength on electroporation efficiency. Methods Mol. Biol. 48, 29–40.
Rols, M. P., Delteil, C., Serin, G., and Teissie, J. (1993) Temperature effects on electroporation of mammalian cells. Nucleic Acids Res. 22, 540.
Zheng, Q. and Chang, D. C. (1991) High-efficiency gene transfection by in situ electroporation of cultured cells. Biochim. Biophys. Acta 1088, 104–110.
Zimmermann, U. (1982) Electric field-mediated fusion and related electrical phenomena. Biochim. Biophys. Acta 694, 227–277.
Pollay, M. (1985) Blood-brain barrier; cerebral edema. Neurosurgery (Wilkins, R. H. and Rengachary, S. S., ed.), McGraw-Hill, New York, pp. 322–342.
Zhu, N., Liggitt, D., Liu, Y., and Debs, R. (1993) Systemic gene expression after intravenous DNA delivery into adult mice. Science 261, 209–211.
Nabel, E. G., Gordon, D., Yang, Z. Y., Xu, L., San, H., Plautz, G. E., Wu, B. Y., Gao, X., Huang, L., and Nabel, G. J. (1992) Gene transfer in vivo with DNAliposome complexes: Lack of autoimmunity and gonadal localization. Hum. Gene Ther. 3, 649–656.
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Nishi, T. et al. (2000). Reporter/Functional Gene Transfer in Rat Brain. In: Jaroszeski, M.J., Heller, R., Gilbert, R. (eds) Electrochemotherapy, Electrogenetherapy, and Transdermal Drug Delivery. Methods in Molecular Medicine, vol 37. Humana Press. https://doi.org/10.1385/1-59259-080-2:339
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DOI: https://doi.org/10.1385/1-59259-080-2:339
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