Abstract
As the chemokine family continues to grow in number, it is becoming increasingly important to determine the distinct function of each molecule. This is particularly important because of the apparent overlapping biological activity of some chemokines in vitro. One approach to determining the unique role of each chemokine is to genetically generate “knockout” mice by homologous recombination. Such mice are selectively deficient in the targeted gene and therefore the immunologic phenotype of these chemokine-deficient mice can be analyzed.
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References
Wynshaw-Boris A. (1996) Model mice and human disease. Nature Genetics 13, 259–260.
Bronson S. K. and Smithies O. (1994) Altering mice by homologous recombination using embryonic stem cells. J. Biol. Chem. 269, 27,155–27,158.
Joyner A. L. (1993) Gene Targeting: A Practical Approach. Oxford University Oxford UK.
Hogan B., Beddington R., Costantini F., and Lacy E. (1994) Manipulating the Mouse Embryo: A Laboratory Manual. Cold Spring Harbor Laboratory Press Cold Spring Harbor, NY.
Cook D. N., Beck M. A., Coffman T. M., Kirby S. L., Sheridan J. F., Pragnell I. B., and Smithies O. (1995) Requirement of MIP-1 alpha for an inflammatory response to viral infection. Science 269, 1583–1585.
Nagasawa T., Hirota S., Tachibana K., Takakura N., Nishikawa S., Kitamura Y., et al. (1996) Defects of B-cell lymphopoiesis and bone-marrow myelopoiesis in mice lacking the CXC chemokine PBSF/SDF-1. Nature 382, 635–638.
Rothenberg M. E., MacLean J. A., Pearlman E., Luster A. D., and Leder P. (1997) Targeted disruption of the chemokine eotaxin partially reduces antigen induced tissue eosinophilia. J. Exp. Med. 185, 785–790.
Lu B., Rutledge B. J., Gu L., Fiorillo J., Lukacs N. W., Kunkel S. L., et al. (1998) Abnormalities in monocyte recruitment and cytokine expression in monocyte chemoattractant protein 1-deficient mice. J. Exp. Med. 187 (4), 601–608.
Zijlstra M., Li E., Sajjadi F., Subramani S., and Jaenisch R. (1989) Germ-line transmission of a disrupted β2-microglobulin gene produced by homologous recombination in embryonic stem cells. Nature 342, 435–438.
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Rothenberg, M.E. (2000). Chemokine Knockout Mice. In: Proudfoot, A.E.I., Wells, T.N.C., Power, C.A. (eds) Chemokine Protocols. Methods in Molecular Biology, vol 138. Humana Press. https://doi.org/10.1385/1-59259-058-6:253
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DOI: https://doi.org/10.1385/1-59259-058-6:253
Publisher Name: Humana Press
Print ISBN: 978-0-89603-722-9
Online ISBN: 978-1-59259-058-2
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