Abstract
Knowledge of a receptor’ pharmacology provides information which is often essential to unraveling the biological function of that receptor. The chemokine system represents a striking example as most receptors in the family bind multiple chemokines, often with complex overlapping selectivities. Two basic methodologies are used to characterize the binding properties of a receptor. In saturation protocols, the binding of a given ligand, usually labeled to allow detection, is determined over a range of concentrations appropriate for that ligand. Competition experiments measure the ability of varying concentrations of an unlabeled ligand to inhibit the binding of a fixed concentration of a labeled ligand. Although saturation protocols have the advantage of theoretical simplicity, they have three significant disadvantages:
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Daugherty, B.L., Siciliano, S.J., Springer, M.S. (2000). Radiolabeled Chemokine Binding Assays. In: Proudfoot, A.E.I., Wells, T.N.C., Power, C.A. (eds) Chemokine Protocols. Methods in Molecular Biology, vol 138. Humana Press. https://doi.org/10.1385/1-59259-058-6:129
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DOI: https://doi.org/10.1385/1-59259-058-6:129
Publisher Name: Humana Press
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Online ISBN: 978-1-59259-058-2
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