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Shiga Toxins

  • Protocol
Bacterial Toxins: Methods and Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 145))

Abstract

The Shiga family of toxins is comprised of a group of genetically and functionally related molecules whose original family member was described 100 yr ago. Up until the early 1980s this group of toxins was little more than a scientific curiosity without a clear role in disease pathogenesis. However, since the discovery of these toxins in E. coli and other Enterobacteriaceae, and their association with identifiable clinical diseases such as hemorrhagic colitis and hemolytic uremic syndrome, their pathophysiological importance has become clear. In this chapter we describe briefly the clinical relevance and mechanisms of actions of the toxins and then focus on the utility of the available methods to purify and assay the toxins.

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Authors and Affiliations

  1. Division of Geographic Medicine and Infectious Diseases, New England Medical Center Hospital, Boston, MA

    David W. K. Acheson

  2. Center for Gastroenterology Research on Absorptive and Secretory Processes, New England Medical Center Hospital, Boston, MA

    Anne V. Kane & Gerald T. Keusch

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  1. David W. K. Acheson
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  3. Gerald T. Keusch
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  1. Research Center Borstel, Germany

    Otto Holst

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© 2000 Humana Press Inc.

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Acheson, D.W.K., Kane, A.V., Keusch, G.T. (2000). Shiga Toxins. In: Holst, O. (eds) Bacterial Toxins: Methods and Protocols. Methods in Molecular Biology™, vol 145. Humana Press. https://doi.org/10.1385/1-59259-052-7:41

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  • DOI: https://doi.org/10.1385/1-59259-052-7:41

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-604-8

  • Online ISBN: 978-1-59259-052-0

  • eBook Packages: Springer Protocols

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