Abstract
The simple sublibrary method described in this chapter allows the detection and rapid isolation of rare clones from bacteriophage λ libraries. The method is based on the ability of PCR to detect clones present in a library at very low frequencies. Clones present at frequencies as low as one in 10,000,000, which would normally be impractical to isolate by conventional probe hybridization, can be rapidly isolated in this way (1). The method can also be used to isolate clones that are initially undetectable by PCR in λ libraries.
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References
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© 1997 Humana Press Inc.
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Lardelli, M. (1997). Generation and PCR Screening of Bacteriophage λ Sublibraries Enriched for Rare Clones (the “Sublibrary Method”). In: White, B.A. (eds) PCR Cloning Protocols. Methods in Molecular Biology™, vol 67. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-483-6:345
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DOI: https://doi.org/10.1385/0-89603-483-6:345
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-0-89603-483-9
Online ISBN: 978-1-59259-553-2
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