Abstract
The enteric nervous system consists of two major plexi, the submucous (Meissner’s) lying between the circular muscle and mucosal layers, and the myenteric (Auerbach’s) between the circular and longitudinal muscle. In recent years, it has become apparent that the chemical coding of the neurons in these two plexi differs significantly within a single species (1) and between species (2). The functional significance of the chemical coding of the enteric neurons is difficult to assess in vivo because of the absence of knowledge about the electrophysiological properties of the neurons and the regulation of neuropeptide/neurotransmitter release.
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Costa, M, Furness, J B, and Liewellyn-Smith, I. (1987) Histochemistry of the enteric nervous system, in Physiology of the Gastrointestinal Tract, 2nd ed (Johnson, L. R, ed.), Raven, New York, pp 1–40.
Pataky, D M, Curtis, S B, and Buchan A M J. (1990) The co-localization of neuropeptides in the enteric nervous system of normal Wistar and non-diabetic BB rats Neuroscience 36, 247–254
Accili, E A, McIntosh, C H S, and Buchan, A. M J (1992) The release of somatostatin-14 from human submucosal ganglia in tissue culture Can J Physiol Pharmacol 71, 619–624
Buchan, A M J, Doyle, A D, and Accili, E A (1990) Canine Jejunal submucosa cultures characterization and release of neural somatostatin Can J Physiol Pharmacol 68, 705–710
Nishi, R and Willard, A L (1985) Neurones dissociated from rat MYP retain differentiated properties when grown in cell culture I Morphologtcal properties and immunocytochemical localization of transmitter candidates Neuroscience 16, 187–199
Gabella, G. (1990) On the plasticity of form and structure of enteric ganglia J Auton Nerv Syst 30, S59–S66
Yurchenko, P D and Furthmayr, H (1984) Self-assembly of basement membrane collagen Biochemistry 23, 1839–1850
Guarnieri, M, Krell, L S, McKhann, G M, Pasternak, G W, and Yamamura, H I (1975) The effects of cell isolation techniques on neuronal membrane receptors Brain Res 93, 337–342.
Meinstrich, M L(1983) Experimental factors involved in separation by centrifugal elutriation, in Cell Separation Methods and Selected Applications, vol 2 (Pretlow, T G. and Pretlow, T P, eds.), Academic, New York, pp33–61
Hartzell, F C and Fambrough, D H(1973) Acetylcholine receptor production into membranes of developing muscle fibres Dev Biol 30, 153–165
Willard, A L and Nishi, R (1985) Neurones dissociated from rat MYP retain differentiated properties when grown in cell culture II. Electrophysiological properties and responses to neurotransmitter candidates Neuroscience 16, 20l–211
Barber, D L, Buchan, A M. J, Leeman, S. E, and Soll, A H (1989) Canine enteric submucosal cultures. transmitter release from neurotensin immunoreactive neurons Neuroscience 32, 245–253
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© 1996 Humana Press Inc., Totowa, NJ
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Accili, E.A., Buchan, A.M.J. (1996). Primary Cell Culture of Human Enteric Neurons. In: Jones, G.E. (eds) Human Cell Culture Protocols. Methods in Molecular Medicine, vol 2. Humana Press. https://doi.org/10.1385/0-89603-335-X:357
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DOI: https://doi.org/10.1385/0-89603-335-X:357
Publisher Name: Humana Press
Print ISBN: 978-0-89603-335-1
Online ISBN: 978-1-59259-586-0
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