Abstract
The osteoclast is a large multinucleate cell formed from the fusion of mono-nuclear precursor cells of hemopoietic origin Unique markers of the osteoclast have been difficult to identify. Widely used techniques, such as histochemical location of tartrate-resistant acid phosphatase, and response to calciotropic hormones do not distinguish effectively between osteoclasts and non-osteo-clastic cells The only unequivocal evidence of osteoclast activity is the ability of the cells to excavate authentic resorption lacunae in vivo and in vitro (1.
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© 1996 Humana Press Inc., Totowa, NJ
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Walsh, C.A., Carron, J.A., Gallagher, J.A. (1996). The Isolation of Osteoclasts from Human Giant Cell Tumors and Long-Term Marrow Cultures. In: Jones, G.E. (eds) Human Cell Culture Protocols. Methods in Molecular Medicine, vol 2. Humana Press. https://doi.org/10.1385/0-89603-335-X:263
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DOI: https://doi.org/10.1385/0-89603-335-X:263
Publisher Name: Humana Press
Print ISBN: 978-0-89603-335-1
Online ISBN: 978-1-59259-586-0
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