Ten-Minute Electrotransformation of Saccharomyces cerevisiae

Abstract

The introduction of exogenous DNA into prokaryotic and eukaryotic cells is one of the most frequently used procedures in the daily laboratory work of a molecular geneticist. In recent years, methods based on high-voltage electric shocks have been established for various species (reviewed in this book). In the case of Saccharomyces cerevisiae, several procedures have been described that emphasize maximal transformation efficiency (1–5). In this case, many parameters had to be optimized that require accurate, and consequently time-consuming, controls.