Abstract
Microdissection and microcloning involve the physical removal of chromosome fragments and the cloning of the collected DNA using specialized microprocedures. Microdissection represents the most direct method for recovery of cloned DNA from an individual chromosome region providing banks of microclones for the genome analysis of the targeted region. A number of chromosome regions have been micro- dissected and successfully microcloned from a wide range of species, including human (1–5), mouse (6–8), Drosophila (9), and, most recently, plants (10).
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References
Bates, G. P, Wainwnght, B J, Williamson, R, and Brown, S. D M (1986) Microdissection and microcloning from the short arm of human chromosome 2. Mol. Cell Biol 6, 3826–3830.
Ludecke, H.-J, Senger, G, Claussen, U, and Horsthemke, B. (1989) Cloning defined regions of the human genome by microdissection of banded chromosomes and enzymatic amplification. Nature 338, 348–350.
Ludecke, H.-J, Senger, G., Claussen, U., and Horsthemke, B. (1990) Construction and characterisation of band-specific DNA libraries Hum Genet 84, 512–516
Buiting, K, Neumann, M, Ludecke, H.-J., Senger, G, Claussen, U, Antich, J., Passarge, E, and Horsthemke, B (1990) Microdissection of the Prader-Willi syndrome chromosome region and identification of potential gene sequences Genomics 6, 521–527
Fielder, W, Clausse, U, Ludecke, H-J, Senger, G, Horsthemke, B, Van Kessel, A. G, Goertzen, W, and Fahsold, R. (1991) New markers for the Neurofibromatosis-2 region generated by microdissection of chromosome 22 Genomics 10, 786–791.
Rohme, D., Fox, H., Herrmann, B., Frischauf, A-M., Edstrom, J-E., Mains, P, Silver, L M, and Lehrach, H (1984) Molecular clones of the mouse t complex derived from microdissected metaphase chromosomes Cell 36, 783–788.
Fisher, E M. C, Cavanna, J S., and Brown, S. D M (1985) Microdissection and microcloning of the mouse X chromosome Proc.Natl Acad Sci. USA 82, 5846–5849
Greenfield, A. J and Brown, S D M. (1987) Microdissection and microcloning from the proximal region of mouse chromosome 7: isolation of clones genetically linked to the Pudgy locus. Genomics 1, 153–158
Scalenghe, F, Turco, E, Edstrom, J-E, Pirotta, V, and Melli, M. L (1981) Microdissection and cloning of DNA from a specific region of Drosophila melanogaster polytene chromosomes. Chromosoma 82, 205–216.
Sandery, M, Forster, J. W, Macadam, S. R., Blunden, R., Neil Jones, R, and Brown, S. D M (1991) Isolation of sequences common to A-and B-chromo-somes of Rye (Secale cereale) by microcloning Plant Mol Biol Reporter 9, 21–30.
Edstrom, J.-E., Kaiser, R., and Rohme, D. (1987) Microcloning of mammalian metaphase chromosomes, in Methods in Enzymology vol 151 (Gottesman, M. M, ed.) Academic, London, UK, pp 503–516
Brown, S. D M. and Greenfield, A. J. (1987) A model to describe the size distribution of mammalian genomic fragments recovered by microcloning. Gene 55, 327–332.
Weith, A., Winking, H., Brachmann, B, Boldyreff, B., and Traut, W. (1987) Microclones from a mouse germ line HSR detect amplification and complex rearrangements of DNA sequences. EMBO J 6, 1295–1300.
Claussen, U., Klein, R, and Schmidt, M. (1986) A pipette method for rapid karyotyping in prenatal diagnosis Prenatal Diagn. 6, 401–408.
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© 1994 Humana Press Inc, Totowa, NJ
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Brown, S.D.M., Carey, A.H. (1994). Chromosome Dissection and Cloning. In: Gosden, J.R. (eds) Chromosome Analysis Protocols. Methods in Molecular Biology™, vol 29. Humana Press. https://doi.org/10.1385/0-89603-289-2:425
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DOI: https://doi.org/10.1385/0-89603-289-2:425
Publisher Name: Humana Press
Print ISBN: 978-0-89603-289-7
Online ISBN: 978-1-59259-516-7
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