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V79 Cytotoxicity Test for Membrane Damage

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In Vitro Toxicity Testing Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 43))

Abstract

The cytotoxic effect of test chemicals in V79 cell culture can be determined by assessing damage to the plasma membrane as determined by a nucleic acid leakage assay.

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References

  1. Fortunati, E. and Bianchi, V. (1989) Plasma membrane damage detected by nucleic acid leakage. Mol. Toxic0l 1, 27–38.

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  2. Walum, E. and Peterson, A. (1982) Tritiated 2-deoxy-D-glucose as a probe for cell membrane permeability studies. Anal. Biochem 120, 8–11.

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  3. Malik, J. K., Schwartz, L. R., and Wiebel, F. J. (1983) Assessment of membrane damage in continuous culture of mammalian cells. Chem. Biol. Interact 45, 29–42.

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  4. Thelestam, M. and Mollby, R (1975) Sensitive assay for detection of toxin-induced damage to the cytoplasmic membrane of human diploid fibroblasts. Infect. Immun. 12, 225–232.

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  5. Bianchr, V. and Fortunati, E (1990) Cellular effects of an anionic surfactant detected in V79 fibroblasts by different cytotoxicity tests. Toxicol. In Vitro 4, 4–16.

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  6. Thelestam, M. and Mollby, R (1975) Determination of toxin-induced leakage of different-sized nucleotides through plasma membrane of human diplord fibroblasts Infect Immun. 11, 640–648

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  7. Thelestam, M. and Möllby, R. (1976) Cytotoxic effects on the plasma membrane of human diploid tibroblasts: a comparative study of leakage tests. Med. Biol. 54, 39–49.

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Author information

Authors and Affiliations

  1. Department of Biology, University of Padova, Italy

    Vera Bianchi

Authors
  1. Vera Bianchi
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Editor information

Editors and Affiliations

  1. University of Hertfordshire, Hatfield, UK

    Sheila O’Hare  & Chris K. Atterwill  & 

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© 1995 Humana Press Inc , Totowa, NJ

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Bianchi, V. (1995). V79 Cytotoxicity Test for Membrane Damage. In: O’Hare, S., Atterwill, C.K. (eds) In Vitro Toxicity Testing Protocols. Methods in Molecular Biology™, vol 43. Humana Press. https://doi.org/10.1385/0-89603-282-5:151

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  • DOI: https://doi.org/10.1385/0-89603-282-5:151

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-282-8

  • Online ISBN: 978-1-59259-530-3

  • eBook Packages: Springer Protocols

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