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Fast Atom Bombardment Mass Spectrometric Characterization of Peptides

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Peptide Analysis Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 36))

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Abstract

The structural characterization of peptides and proteins is an integral part of biotechnology/pharmaceutical industry research in order to develop novel therapeutic agents through the production of these compounds by synthesis and/or recombinant DNA techniques. The most commonly used methods are the indirect approach through the translation of the DNA sequence of the gene coding for the protein and the automated stepwise Edman degradation. Nevertheless, the former can introduce inaccuracies either from mistakes in reading DNA sequencing gels or from posttranslational modifications, and the latter cannot be used for N-terminally blocked proteins/peptides and certain modified amino acids. Recent developments in desorption ionization techniques and instrumentation have made mass spectrometry a complementary alternative, and in several cases, an effective method of choice over conventional analytical methods employed in the evaluation of a solid-phase synthetic peptide or a recombinant product.

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Author information

Authors and Affiliations

  1. Schering-Plough Research Institute, Kenilworth, NJ

    P. R. Das & B. N. Pramanik

Authors
  1. P. R. Das
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  2. B. N. Pramanik
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Editor information

Editors and Affiliations

  1. University of Florida, Gainesville, FL

    Ben M. Dunn

  2. Bachem Bioscience, King of Prussia, PA

    Michael W. Pennington

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© 1994 Humana Press Inc., Totowa, NJ

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Das, P.R., Pramanik, B.N. (1994). Fast Atom Bombardment Mass Spectrometric Characterization of Peptides. In: Dunn, B.M., Pennington, M.W. (eds) Peptide Analysis Protocols. Methods in Molecular Biology, vol 36. Humana Press. https://doi.org/10.1385/0-89603-274-4:85

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  • DOI: https://doi.org/10.1385/0-89603-274-4:85

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-274-3

  • Online ISBN: 978-1-59259-523-5

  • eBook Packages: Springer Protocols

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