Abstract
The screening of a λgt11 library with monoclonal antibodies described here is a relatively uncomplicated procedure, but it requires a little introduction nevertheless. For simplicity, it is assumed that you have in your possession a library that is ready to screen, i.e., either a library bought from a company or a library that you have made. Construction of a λgt11 library is described in Volume 4, Chapter 19.
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References
de Wet, J. R., Fukushima, H., Dewji N., Wilcox, E., O’Brien, J. S., and Helinski, D. R. (1984) Chromogenic immunodetection of human serum albumin and α-L-fucosidase clones in a human hepatoma cDNA expression library. DNA 3, 437–447.
Young, R. A. and Davis, R. W. (1983) Efficient isolation of genes by using antibody probes. Proc. Nat. Acad. Sci. USA 80, 1194–1198.
Young, R. A. and Davis, R. W. (1983) Yeast RNA polymerase II genes: Isolation with antibody probes. Science 222, 778–782.
Maniatis, T., Fritsch, E. F., and Sambrook, J. (1982) Molecular Cloning, A Laboratory Manual Cold Spring Harbor, Cold Spring Harbor, NY.
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© 1992 The Humana Press, Inc., Totowa, NJ
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Webster, D.F., Melvin, W.T., Burke, M.D., Carr, F.J. (1992). Screening of λ gt11 cDNA Libraries Using Monoclonal Antibodies. In: Manson, M.M. (eds) Immunochemical Protocols. Methods in Molecular Biology, vol 10. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-204-3:451
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DOI: https://doi.org/10.1385/0-89603-204-3:451
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-0-89603-204-0
Online ISBN: 978-1-59259-497-9
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