Skip to main content
SpringerLink
Log in

Herpes simplex virus infection of human spermatozoa correlates with decreased frequency of blastocyst formation and frequency of embryo implantation during in vitro fertilization

  • Medical Embryology
  • Published:
Russian Journal of Developmental Biology Aims and scope Submit manuscript

Abstract

We have conducted a comparative analysis of developing human embryos in the course of in vitro fertilization (IVF) as a method of sterility treatment of two groups of patients: herpes simplex virus (HSV) was detected in the fraction of motile sperm of male partners in group I (n = 28) and no HSV was found in sperm in group II (n = 103). We assessed number of fertilized ova, embryos during cleavage, and blastocysts as well as such parameters as frequency of implantation and frequency of pregnancy in IVF cycles. It was established that the presence of HSV in spermatozoa did not affect the efficiency of fertilization or cleavage of zygotes. At the same time, in cases of virus-infected male gametes, the frequency of blastocyst formation was two times less (p = 0.015), and the frequency of embryo implantation and pregnancy was, on average, five times lower (p = 0.01 and p = 0.016, respectively). Based on the obtained results, a conclusion was made about the negative influence of HSV-virus in male gametes at the early stages of embryo development and the frequency of achievement of pregnancy in respect to the methods of assisted reproductive technologies (ART).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Abdulmedzhidova, A.G., Kurilo, L.F., Shileiko, L.V., et al., Asymptomatic Form of Genital Herpes and Infertility in Men, Urologiya, 2007, no. 3, pp. 56–59.

  • Baranov, V.S. and Kuznetsova, T.V., Tsitogenetika embrional’nogo razvitiya cheloveka: Nauchno-prakticheskie aspekty (Cytogenetics of Human Embryonic Development: Scientific and Practical Aspects), St. Petersburg: NL, 2007.

    Google Scholar 

  • Bezold, G., Politch, J.A., Kiviat, N.B., et al., Prevalence of Sexually Transmissible Pathogens in Semen from Asymptomatic Male Infertility Patients with and Without Leukocytospermia, Fertil. Steril., 2007, vol. 87, no. 5, pp. 1087–1097.

    Article  PubMed  Google Scholar 

  • Bocharova, E.N., Bragina, E.E., Gusak, Yu.K., et al., Spontaneous Abortion, Failures in Using Reproductive Technologies, and Herpes Infection of Sperms, Androl. Genit. Khir., 2006, no. 1, pp. 59–65.

  • Bocharova, E.N., Zavalishina, L.E., Bragina, E.E., et al., Detection of Herpes Simplex Virus Genomic DNA in Spermatozoa of Patients with Fertility Disorders by in situ Hybridization, Doklady Biol. Sci., 2007, vol. 412, pp. 82–86.

    Article  CAS  Google Scholar 

  • Bragina, E.E., Abdulmalikov, R.A., Kurilo, L.F., et al., Identification of Sperms Infected with Herpes Simplex Virus, Vestn. Dermatol. Venerol., 2000, no. 5, pp. 18–22.

  • Chenet-Monte, C., Mohammad, F., Celluzzi, C.M., et all., Herpes Simplex Virus Gene Products Involved in the Induction of Chromosomal Aberrations, Virus Res., 1986, vol. 6, no. 3, pp. 245–260.

    Article  PubMed  CAS  Google Scholar 

  • Clouston, H.J., Herbert, M., Fenwick, J., et al., Cytogenetic Analysis of Human Blastocysts, Prenat. Diag., 2002, vol. 22, no. 12, pp. 1143–1152.

    Article  Google Scholar 

  • Csata, S. and Kulcsar, G., Virus-Host Studies in Human Seminal and Mouse Testicular Cells, Acta Chir. Hung., 1991, vol. 32, pp. 83–90.

    PubMed  CAS  Google Scholar 

  • Dejucq, N. and Jegou, B., Viruses in the Mammalian Male Genital Tract and Their Effects on the Reproductive System, Microbiol. Mol. Biol., 2001, vol. 65, pp. 208–231.

    Article  CAS  Google Scholar 

  • Deture, F.A., Drylie, D.M., Kaufman, H.E., and Centifanto, Y.M., Herpesvirus Type 2: Isolation from Seminal Vesicle and Testes, Urology, 1976, vol. 7, pp. 541–544.

    Article  PubMed  CAS  Google Scholar 

  • Hwang, C.B. and Shillitoe, E.J., DNA Sequence of Mutations Induced in Cells by Herpes Simplex Virus Type-1, Virology, 1990, vol. 178, no. 1, pp. 180–188.

    Article  PubMed  CAS  Google Scholar 

  • Jamieson, M.E., Coutts, J.R., and Connor, J.M., The Chromosome Constitution of Human Preimplantation Embryos Fertilized in vitro, Hum. Reprod., 1994, vol. 9, no. 4, pp. 709–715.

    PubMed  CAS  Google Scholar 

  • Klimova, R.R., Chichev, E.V., Naumenko, V.A., et al., Herpes Simplex Virus and Cytomegalovirus in Semen of Men: the Herpes Simplex Virus Is More Common in Idiopathic Infertility and Correlates with a Reduction in Sperm Characteristics, Vopr. Virusol., 2010, vol. 55, no. 1, pp. 27–31.

    PubMed  CAS  Google Scholar 

  • Kotronias, D. and Kapranos, N., Detection of Herpes Simplex Virus DNA in Human Spermatozoa by in situ Hybridization Technique, In vivo, 1998, vol. 12, pp. 391–394.

    PubMed  CAS  Google Scholar 

  • Liu, M., Schmidt, E.E., and Halford, W.P., ICP0 Dismantles Microtubule Networks in Herpes Simplex Virus-Infected Cells, PLoS One, 2010, vol. 8, nos. 5–6, p. e10975.

    Article  Google Scholar 

  • Moore, D.E., Ashley, R.L., Zarutskie, P.W., et al., Transmission of Genital Herpes by Donor Insemination, J. Amer. Med. Assoc., 1989, vol. 261, pp. 3441–3443.

    Article  CAS  Google Scholar 

  • Naumenko, V.A., Klimova, R.R., Kurilo, L.F., et al., Detection of Herpes Simplex Virus in Male Germ Cells during Experimental Infection of Testis Organ Cultures and in Ejaculate of Male Patients with Fertility Disorders, Akusher. Ginekol., 2010, no. 3, pp. 42–46.

  • Van Landuyt, L., De Vos, A., Joris, H., et al., Blastocyst Formation in in vitro Fertilization Versus Intra Cytoplasmic Sperm Injection Cycles: Influence of the Fertilization Procedure, Fertil. Steril., 2005, vol. 83, pp. 1397–1403.

    Article  PubMed  Google Scholar 

  • Van Royen, E., Mangelschots, K., De Neubourg, D., et al., Characterization of a Top Quality Embryo, a Step Towards Single-Embryo Transfer, Hum Reprod., 1999, vol. 14, no. 9, pp. 2345–2349.

    Article  PubMed  Google Scholar 

  • Wald, A., Matson, P., Ryncarz, A.., and Corey, L., Detection of Herpes Simplex Virus DNA in Semen of Men with Genital HSV-2 Infection, Sex. Transm. Dis., 1999, vol. 26, pp. 1–3.

    Article  PubMed  CAS  Google Scholar 

  • WHO Laboratory Manual for the Examination of Human Semen and Sperm-Cervical Mucus Interaction, 5th ed., Cambridge: Cambridge Univ. Press, 2010.

Download references

Author information

Authors and Affiliations

  1. Ivanovsky Research Institute of Virology Federal State Institution, Ministry of Health and Social Development of the Russian Federation, ul. Gamalei 16, Moscow, 123098, Russia

    A. S. Tsibizov, A. G. Abdulmedzhidova, Z. S. Gadzhieva & A. A. Kushch

  2. Reproduction Department, Moscow Regional Scientific-Research Institute for Obstetrics and Gynecology State Healthcare Institution, Vtoroi Botkinskii proezd 5, korp. 5, Moscow, 125101, Russia

    K. V. Krasnopol’skaya

Authors
  1. A. S. Tsibizov
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. G. Abdulmedzhidova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. K. V. Krasnopol’skaya
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Z. S. Gadzhieva
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. A. Kushch
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. S. Tsibizov.

Additional information

Original Russian Text © A.S. Tsibizov, A.G. Abdulmedzhidova, K.V. Krasnopol’skaya, Z.S. Gadzhieva, A.A. Kushch, 2011, published in Ontogenez, 2011, Vol. 42, No. 6, pp. 403–425.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tsibizov, A.S., Abdulmedzhidova, A.G., Krasnopol’skaya, K.V. et al. Herpes simplex virus infection of human spermatozoa correlates with decreased frequency of blastocyst formation and frequency of embryo implantation during in vitro fertilization. Russ J Dev Biol 42, 397–401 (2011). https://doi.org/10.1134/S1062360411060063

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1062360411060063

Keywords

Search

Navigation