Abstract
Body fluid homeostasis undergoes significant changes during hypokinesia (diminished movement). Understanding of fluid transfer between body fluid compartments and its regulating mechanisms was the aim of this study for disclosing impaired fluid retention during hypokinesia (HK).Studies were done on 12 male Macaca mulatta (rhesus monkeys) aged three to five years (5.15 to 6.56 kg) during 90 days period of pre-HK and 90 days period of HK. All primates were divided equally into two groups: vivarium control primates (VCP) and hypokinetic primates (HKP). Hypokinetic primates were kept for 90 days in small individual cages that restricted their movements in all directions without hindering food and fluid intakes. Control primates were housed in individual cages without their movements being restricted.Total body fluid (TBF), intracellular fluid volume (IFV) and circulating plasma volume (CPV) decreased significantly (p < 0.05), while extracellular fluid volume (EFV) and interstitial fluid volume (IsFV) decreased significantly (p < 0.05) at the initial seven days of the HK period and after the 7th day increase progressively in HKP compared with their pre-HK values and their respective control values in VCP. Fluid excretion, urinary and plasma sodium (Na) and potassium (K) levels increased significantly (p < 0.05), while fluid intake and fluid retention decreased significantly (p < 0.05) in HKP compared with their pre-HK values and their respective controls in VCP. The measured parameters did not change in VCP compared with their pre-HK values.It was concluded that decreased IFV and CPV may demonstrate fluid depletion, while marked increase of fluid loss despite of fluid depletion may demonstrate impair fluid retention during HK. Dissociation between fluid loss and fluid depletion maydemonstrate the presence of reduced fluid retention as the mechanism ofdevelopment of fluid depletion during HK.
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References
Balakhovskiy IS, Kiselev RK, Kaplan MA, Serada MG. Changes in total body potassium, haemoglobin, and bromine space in the crew. Kosmicheskaya Biol 1978; 12: 11–15.
Grigor'yev AI. Regulation of Fluid-electrolyte Metabolism and Renal Function in Man during Cosmic Flights, PhD Thesis, 'Interkosmos' Council. Academy of Sciences USSR and Directorate of Cosmic Biology and Medicine, Ministry of Health USSR, Moscow, Russia, 1980; p. 289.
Grigor'yev AI, Dorokhova BR, Kozyrevskaya GI. Water salt metabolism and functional state of the kidney during bed rest of varying duration, Fiziol. Cheloveka 1979; 65: 660–669.
Grigor'yev AI, Lichardus BL, Lobachik VL. Analysis of body fluid volumes in animals by using mathematical methods. Physiologists 1983; 26: 28–29.
Krotov VP. Study of water-mineral metabolism during restriction of motor activity. Kosmicheskaya Biol 1972; 6: 66–74.
Krotov VP. Kinetics and Regulation of Fluid and Electrolyte Metabolism in Animals and Human beings during Prolonged Hypokinesia, PhD Thesis, 'Interkosmos' Council. Academy of Sciences USSR and Directorate of Kosmic Biology and Medicine, Ministry of Health USSR, Moscow, Russia, 1978.
Krupina TN, Mikhaylovskiy GP, Tizul A Ya. Hypokinesia as an Unusual Stress Stimulus in Adaptation of Muscular Activity and Hypokinesia. Meditsina Press, Novosibirsk, 1970; pp. 94–94.
Moore FD, Olesen KH. The Body Cell Mass and Its Supporting Environment: Body Composition in Health and Disease. WB Saunders Co, Philadelphia and London, 1963; p. 535.
Sobocinska J. The effect of prolonged immobilization on diuresis and water intake in rats. Space Life Scin 1973; 4: 200–203.
Volozhin AV. Pathogensis of Disturbances of Calcium Metabolism in Mineralized Tissues during Long-term Hypokinesia, Ph.D. Thesis, 'Interkosmos' Council. Academy of Sciences USSR and Directorate of Kosmic Biology and Medicine, Ministry of Health USSR, Moscow, Russia, 1978.
Zorbas YG, Bobylev VR. General resistance of organism of rats under hypokinesia. In: Proceedings 2th European Symposium on Life Sciences Research in Space. Poz-Whan, Germany (ESA SP-212-August 1984), 1984: 203–206.
Zorbas YG, Ivanov AL, Federenko YF. Electrolyte and water contents in organs and tissues of rats during and after exposure to prolonged restriction of motor activity. Rev Esp Fisiol (J Physiol Biochem) 1995; 51: 155–162.
Zorbas YG, Matvedev IN, Tantareli RK. Hyperhydration effect on athletes physiological reactions to maximum physical loads. In: Hermans GPH, Mosterd WL, eds, Sports, Medicine and Health, ICR–921. Elsevier Science Publisher, Holland, 1990: 636–641.
Zorbas YG, Yaroshenko YY, Kuznetsov NK et al. Electrolyte concentration in skeletal muscles and plasma of rats during and after exposure to hypokinesia and hyperhydration. Physiol Chem Phys And Med NMR 1997; 29: 243–259.
Zorbas YG, Yarullin VL, Naexu KA et al. Water and electrolyte excretion in rats during prolonged restriction of motor activity and chronic hyperhydration. Physiol Chem Phys And NMR 1997; 30: 167–184.
Zorbas YG, Kakurin VJ, Ytaroshenko YY et al. Daily magnesium supplements effect on magnesium deficiency in rats during prolonged restriction of motor activity. Metabolism 1998; 47: 903–907.
Zorbas YG, Kakurin VJ, Afonin VB et al. Calcium measurements in calcium supplemented primates during and after hypokinesia in establishing calcium deficiency during prolonged hypokinesia. Biological Trace Element Research 2000; 75: 113–131.
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Zorbas, Y.G., Kakurin, V.J., Denograndov, S.D. et al. Fluid distribution measurement between body compartments in primates in disclosing fluid retention during prolonged hypokinesia. Int Urol Nephrol 34, 303–310 (2002). https://doi.org/10.1023/A:1024482801322
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DOI: https://doi.org/10.1023/A:1024482801322