Abstract
One hundred and ninety five strains of fungi were observed during freezing and thawing using a cryogenic light microscope. There was no obvious link between taxonomic position and their morphological response to freezing and thawing. The viability of seven of these strains was examined following freezing and thawing in the presence or absence of the cryoprotectants glycerol and dimethyl sulphoxide. Intracellular ice and hyphal shrinkage were not necessarily lethal events, but in many cases they affected the rate and quality of growth. Both cryoprotectants reduced shrinkage, shifted the cooling rate where intracellular ice formed in many cases, and improved the recovery of strains. The results presented aid the development of successful cryopreservation protocols.
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Smith, D., Thomas, V.E. Cryogenic light microscopy and the development of cooling protocols for the cryopreservation of filamentous fungi. World Journal of Microbiology and Biotechnology 14, 49–57 (1997). https://doi.org/10.1023/A:1008820432471
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DOI: https://doi.org/10.1023/A:1008820432471