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Molecular weight-dependent electron transfer capacities of dissolved organic matter derived from sewage sludge compost

  • SOILS, SEC 2 • GLOBAL CHANGE, ENVIRON RISK ASSESS, SUSTAINABLE LAND USE • RESEARCH ARTICLE
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Abstract

Purpose

Dissolved organic matter (DOM) has been shown to be an efficient electron transfer facilitator in biogeochemical reactions due to its ability to mediate redox reactions. It has been known that various fractions of DOM differed in their chemical and biogeochemical behaviors in environment. However, there has been relatively little work directed at predicting the dependence of redox properties of DOM on its fractions.

Materials and methods

DOM was extracted from sewage sludge compost. Freeze-dried DOM was grouped into three fractions of different molecular sizes (<3,500, 3,500–14,000, and >14,000 Da) using dialysis bags (Spectra/Por 3 and 4, Spectrum Industries, California, US). Cycle voltammetry was used to investigate the redox behavior of the fractions. Chronoamperometry was employed to study their electron accepting capacities and electron donating capacities by applying fixed positive or negative potential to the working electrode in a conventional three-electrode cell. Fourier-transform infrared and three-dimensional excitation/emission matrix fluorescence spectroscopies were used to determine the functional groups in the fractions. Shewanella putrefaciens 200 (SP200) and Klebsiella pneumoniae L17 (L17) were used for all microbial iron(III) reduction experiments.

Results and discussion

Electrochemical methods show that the electron transfer capacity (ETC) of DOM depends on its molecular weight, and ETC is in the order of high-molecular weight DOM (H-DOM) > moderate-molecular weight DOM > low-molecular weight DOM. The same trend is discovered in the DOM-stimulated iron(III) oxide bioreduction where DOM fractions act as electron shuttles transferring electrons from the Fe(III)-reducing bacteria to the iron oxide. Both spectroscopic and cyclic voltammogram assays show the highest abundance of redox moieties associated to H-DOM, which is possibly responsible for its strongest electron-shuttling ability.

Conclusions

DOM has a wide molecular weight (MW) distribution due to the complexity of its chemical composition and structure. In addition to structural variations, DOM fractions with different MW have different redox properties and electron-shuttling capacities in microbial Fe(III) reduction. The results are of great significance for further studies on DOM geochemical behavior in environment.

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References

  • Aeschbacher M, Sander M, Schwarzenbach RP (2010) Novel electrochemical approach to assess the redox properties of humic substances. Environ Sci Technol 44:97–93

    Article  Google Scholar 

  • Amon R, Benner R (1996) Bacterial utilization of different size classes of dissolved organic matter. Limnol Oceanogr 41:41–51

    Article  CAS  Google Scholar 

  • Amy GL, Sierka RA, Bedessem J (1992) Molecular size distribution of dissolved organic matter. J Am Water Works Assoc 84:67–75

    CAS  Google Scholar 

  • Bauer M, Heitmann T, Macalady DL (2007) Electron transfer capacities and reaction kinetics of peat dissolved organic matter. Environ Sci Technol 4:139–145

    Article  Google Scholar 

  • Burgos WD, Pisutpaisal N, Tuntoolavest M, Chorover J, Unz RF (2000) Biodegradation of 1-naphthol in the presence of humic acid. Environ Eng Sci 17:343–351

    Article  CAS  Google Scholar 

  • Chen J, Baohua G, LeBoeuf EJ, Pan H, Dai S (2002) Spectroscopic characterization of the structural and functional properties of natural organic matter fractions. Chemosphere 48:59–68

    Article  CAS  Google Scholar 

  • Chen G, Lin C, Chen L, Yang H (2010) Effect of size-fractionation dissolved organic matter on the mobility of prometryne in soil. Chemsphere 79:1046–1056

    Article  CAS  Google Scholar 

  • Chian ESK (1977) Stability of organic matter in landfill leachates. Water Res 11:225–232

    Article  CAS  Google Scholar 

  • Chin YP, Aiken G, O’Loughlin E (1994) Molecular weight, polydispersity, and spectroscopic properties of aquatic humic substances. Environ Sci Technol 28:1853–1858

    Article  CAS  Google Scholar 

  • Coble PG (1996) Characterisation of marine and terrestrial dissolved organic matter in seawater using excitation-emission matrix spectroscopy. Mar Chem 51:325–346

    Article  CAS  Google Scholar 

  • Cory R, Mcknight D (2005) Fluorescence spectroscopy reveals ubiquitous presence of oxidized and reduced quinones in dissolved organic matter. Environ Sci Technol 39:8142–8149

    Article  CAS  Google Scholar 

  • Fredrickson JK, Zachara JM, Kennedy DW, Duff MC, Gorby YA, Li SW, Krupka KM (2000) Reduction of U(VI) in goethite (α-FeOOH) suspensions by a dissimilatory metal-reducing bacterium. Geochim Cosmochim Acta 64:3085–3098

    Article  CAS  Google Scholar 

  • Heitmann T, Goldhammer T, Beer J (2007) Electron transfer of dissolved organic matter and its potential significance for anaerobic respiration in a northern bog. Glob Change Biol 13:1771–1785

    Article  Google Scholar 

  • Her N, Amy G, McKnight D (2003) Characterization of DOM as a function of MW by fluorescence EEM and HPLC-SEC using UVA, DOC, and fluorescence detection. Water Res 37:4295–4303

    Article  CAS  Google Scholar 

  • Huang DY, Zhuang L, Cao WD, Xu W, Zhou SG, Li FB (2010) Comparison of dissolved organic matter from sewage sludge and sludge compost as electron shuttles for enhancing Fe(III) bioreduction. J Soil Sediments 10:722–729

    Article  CAS  Google Scholar 

  • Huo SL, Xi BD, Yu HC (2008) Characteristics of dissolved organic matter (DOM) in leachate with different landfill ages. J Environ Sci 20:492–498

    Article  CAS  Google Scholar 

  • Kaiser K, Guggenberger G, Haumaier L, Zech W (2002) The composition of dissolved organic matter in forest solutions: changes induced by seasons and passage through the mineral soil. Org Geochem 33:307–318

    Article  CAS  Google Scholar 

  • Kalbitz K, Solinger S, Park JH, Michalzik B, Matzner E (2000) Control on the dynamics of dissolved organic matter in soils: a review. Soil Sci 165:277–304

    Article  CAS  Google Scholar 

  • Kang KH, Shin HS, Park H (2002) Characterization of humic substances present in landfill leachates with different landfill ages and its implications. Water Res 36:4023–4032

    Article  CAS  Google Scholar 

  • Kappler A, Haderlei SB (2003) Natural organic matters reductant for chlorinated aliphatic pollutants. Environ Sci Technol 37:2714–2719

    Article  CAS  Google Scholar 

  • Kilduff JE, Karanfil T, Chin YP (1996) Adsorption of natural organic polyelectrolytes by activated carbon: A size-exclusion chromatography study. Environ Sci Technol 30:1336–1343

    Article  CAS  Google Scholar 

  • Li XM, Zhou SG, Li FB, Wu CY, Zhuang L, Xu W, Liu L (2009) Fe(III) oxide reduction and carbon tetrachloride dechlorination by a newly isolated Klebsiella pneumoniae strain L17. J Appl Microbiol 106:130–139

    Article  CAS  Google Scholar 

  • Lovely DR, Coates JD, Bluntharris EL, Phillips EP, Woodward JC (1996) Humic substances as electron acceptors for microbial respiration. Nature 382:445–448

    Article  Google Scholar 

  • Lovley DR, Blunt-Harris EI (1999) Role of humic-bound iron as an electron transfer agent in dissimilatory Fe(III) reduction. Appl Environ Microbiol 65:4252–4254

    CAS  Google Scholar 

  • McCarthy M, Hedges J, Benner R (1996) Major biochemical composition of dissolved high-molecular-weight organic matter in seawater. Mar Chem 55:281–297

    Article  CAS  Google Scholar 

  • Miano TM, Senesi N (1992) Synchronous excitation fluorescence spectroscopy applied to soil humic substances chemistry. Sci Total Environ 117:41–51

    Article  Google Scholar 

  • Michalzik B, Matzner E (1999) Fluxes and dynamics of dissolved organic nitrogen and carbon in a spruce (Picea abies Karst.) forest ecosystem. Eur J Soil Sci 50:579–590

    Article  Google Scholar 

  • Newcombe G, Drikas M, Hayes R (1997) Influence of characterized natural organic material on activated carbon adsorption: II. Effect of pore volume distribution and adsorption of 2-methylisoborneol. Water Res 31:1065–1073

    Article  CAS  Google Scholar 

  • Nurmi J, Tratnyek PG (2002) Electrochemical properties of natural organic matter (NOM), fractions of NOM, and Model biogeochemical electron shuttles. Environ Sci Technol 36:617–624

    Article  CAS  Google Scholar 

  • Picardal F, Arnold RG, Huey BB (1995) Effects of electron donor and acceptor conditions on reductive dehalogenation of tetrachloromethane by Shewanella putrefaciens 200. Appl Environ Microbiol 61:8–12

    CAS  Google Scholar 

  • Scott DT, McKnight DM, Blunt-Harris EL, Kolesar SE, Lovley DR (1998) Quinone moieties act as electron acceptors in the reduction of humic substances by umics-reducing microorganisms. Environ Sci Technol 32:2984–2989

    Article  CAS  Google Scholar 

  • Silverstein RM, Bassler GC, Morrill TC (1991) Spectrometric identification of organic compounds. Wiley, New York, pp 109–130

    Google Scholar 

  • Song NH, Chen L, Yang H (2008) Effect of dissolved organic matter on mobility and activation of chlorotoluron in soil and wheat. Geoderma 146:344–352

    Article  CAS  Google Scholar 

  • Steelink C (1985) Implication of elemental characteristics of humic substances. Humic substances in soil, sediment and water. John Wiley, New York, pp 457–476

    Google Scholar 

  • Wolf M, Kappler A, Jiang J, Meckenstock RU (2009) Effects of humic substances and quinones at low concentrations on ferrihydrite reduction by Geobacter metallireducens. Environ Sci Technol 43:5679–5685

    Article  CAS  Google Scholar 

  • Yuan T, Yuan Y, Zhou SG, Li FB, Liu Z, Zhuang L (2011) A rapid and simple electrochemical method for evaluating the electron transfer capacities of dissolved organic matter. J Soils Sediments 11:467–473

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This study was supported jointly by the National Natural Science Foundation of China (numbers 41101211, 41071157, and 40801119), the Natural Science Foundation of Guangdong Province, China (number 10151065003000004), and the Foundation for Excellent Young Scientist in Guangdong Academy of Sciences (number rcjj201101).

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Authors and Affiliations

  1. Guangdong Institute of Eco-environmental and Soil Sciences, 808 Tianyuan Road, Guangzhou, Guangdong Province, 510650, People’s Republic of China

    Yong Yuan, Shungui Zhou, Tian Yuan, Li Zhuang & Fangbai Li

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  1. Yong Yuan
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  2. Shungui Zhou
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  3. Tian Yuan
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  4. Li Zhuang
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  5. Fangbai Li
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Corresponding author

Correspondence to Shungui Zhou.

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Responsible editor: Huijun Zhao

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ESM 1

Iron(III) reduction driven by Klebsiella pneumoniae L17, FT-IR spectra, and elemental composition of the DOM. Table of electrochemical parameters obtained from CV analysis (DOC 354 kb)

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Yuan, Y., Zhou, S., Yuan, T. et al. Molecular weight-dependent electron transfer capacities of dissolved organic matter derived from sewage sludge compost. J Soils Sediments 13, 56–63 (2013). https://doi.org/10.1007/s11368-012-0585-y

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  • DOI: https://doi.org/10.1007/s11368-012-0585-y

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