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Protective effects of anthocyanin against apoptosis and oxidative stress induced by arsanilic acid in DF-1 cells

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Abstract

Anthocyanin is a natural plant pigment that acts as an antioxidant and scavenges free radicals. This study aimed to investigate the potential protective role of nightshade anthocyanin (NA), a natural flavonoid compound, against the arsanilic acid (ASA)-induced cell death of DF-1 cells. DF-1 cells were initially exposed to ASA, and then NA was applied to the treated cells. Cell viability, intracellular reactive oxygen species (ROS), mitochondrial membrane potential (MMP), and apoptosis were examined. Results showed that NA inhibited the ASA-induced decrease in cell viability, increase in ROS, and loss of MMP in DF-1 cells. Moreover, caspase-3 activation was inhibited by ASA supplementation and NA attenuated the ASA-induced increase in the percentage of apoptotic cells. In summary, our study suggested that NA can enhance ASA-induced cytotoxicity and apoptosis, thereby providing a basis for the molecular mechanisms of NA-mediated protection.

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Acknowledgements

The project was supported by the National Key R&D Program (2016YFD0501208, 2016YFD0501007), National Nature Science Foundation of China (No. 31872535), Shandong Natural Science Foundation of China (ZR2018MC027, ZR2016CQ29) and Funds of Shandong “Double Tops” Program.

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  1. Ning Xie, Na Geng and Dong Zhou contributed equally to this work.

Authors and Affiliations

  1. College of Veterinary Medicine, Shandong Agricultural University, Tai’an, 271018, Shandong, China

    Ning Xie, Kangping Liu & Jianzhu Liu

  2. Research Center for Animal Disease Control Engineering, Shandong Agricultural University, Tai’an, 271018, Shandong, China

    Na Geng & Yuliang Xu

  3. Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, Tai’an, 271018, Shandong, China

    Ning Xie

  4. College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling, 712100, China

    Dong Zhou & Yongxia Liu

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  1. Ning Xie
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Correspondence to Yongxia Liu or Jianzhu Liu.

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Xie, N., Geng, N., Zhou, D. et al. Protective effects of anthocyanin against apoptosis and oxidative stress induced by arsanilic acid in DF-1 cells. Mol Biol Rep 46, 301–308 (2019). https://doi.org/10.1007/s11033-018-4472-5

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  • DOI: https://doi.org/10.1007/s11033-018-4472-5

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