Summary
DNA segments carrying rRNA genes of Mycoplasma capricolum have been cloned and characterized by restriction endonuclease mapping, DNA-RNA hybridization and nucleotide sequencing. The M. capricolum genome has two sets of rRNA gene clusters, where the arrangement is in the order of (5′)16S-23S-5S(3′). The spacer region between 16S and 23S rDNA is extremely rich in AT and does not carry any tRNA genes.
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References
Bram RJ, Young RJ, Steitz JA (1980) The ribonuclease III site flanking 23S sequences in the 30S ribosomal precursor RNA of E. coli. Cell 19:393–401
Brosius J, Dull TJ, Sleeter DD, Noller HF (1981) Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli. J Mol Biol 148:107–127
Grunstein M, Hogness DS (1975) Colony hybridization; a method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci USA 72:3961–3965
Hori H, Sawada M, Osawa S, Murano K, Ishikura H (1981) The nucleotide sequence of 5S rRNA from Mycoplasma capricolum. Nucl Acids Res 9:5407–5410
Ikemura T, Nomura M (1977) Expression of spacer tRNA genes in ribosomal RNA transcription units carried by hybrid Col El plasmids in E. coli. Cell 11:779–793
Iwami M, Muto A, Yamao F, Osawa S (1984) Nucleotide sequence of rrnB 16S ribosomal RNA gene from Mycoplasma capricolum. Mol Gen Genet 196:317–322
Kawauchi Y, Muto A, Osawa S (1982) The protein composition of Mycoplasma capricolum. Mol Gen Genet 188:7–11
Loughney K, Lund E, Dahlberg JE (1982) tRNA genes are found between the 16S and 23S rRNA genes in Bacillus subtilis. Nucl Acids Res 10:1607–1624
Lund E, Dahlberg JE, Lindahl L, Jaskunas SR, Dennis PP, Nomura M (1976) Transfer RNA genes between 16S and 23S rRNA genes in rRNA transcription units of E. coli. Cell 7:165–177
Maniloff J, Morowitz HJ (1972) Cell biology of the mycoplasmas. Bacteriol Rev 36:263–290
Maxam AM, Gilbert W (1977) A new method for sequencing DNA. Proc Natl Acad Sci USA 74:560–564
Morgan EA, Ikemura T, Nomura M (1977) Identification of spacer tRNA genes in individual ribosomal RNA transcription units of Escherichia coli. Proc Natl Acad Sci USA 74:2710–2714
Neimerk HC (1970) Division of mycoplasmas into subgroups. J Gen Microbiol 63:249–263
Ogasawara N, Moriya S, Yoshikawa H (1983) Structure and organization of rRNA operons in the region of the replication origin of the Bacillus subtilis chromosome. Nucl Acids Res 11:6301–6318
Oka A, Sugisaki H, Takanami M (1981) Nucleotide sequence of the kanamycin resistance transposon Tn903. J Mol Biol 147:217–226
Razin S (1978) The Mycoplasmas. Microbiol Rev 42:414–470
Ryan JL, Morowitz HJ (1969) Partial pruification of native rRNA and tRNA cistrons from Mycoplasma sp. (KID). Proc Natl Acad Sci USA 63L1282–1289
Sawada M, Osawa S, Kobayashi H, Hori H, Muto A (1981) The number of ribosomal RNA genes in Mycoplasma capricolum. Mol Gen Genet 182:502–504
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
Stanbridge EJ, Reff EM (1979) The molceular biology of mycoplasmas. In: Barile MF, Razin S (eds) The Mycoplasmas 1, Academic Press, New York, p 157–185
Tomioka N, Shinozaki K, Sugiura M (1981) Molecular cloning and characterization of ribosomal RNA genes from a blue green alga, Anacystis nidulans. Mol Gen Genet 184:359–363
Vogelstcin B, Gillespie D (1979) Preparative and analytical purification of DNA from agarose. Proc Natl Acad Sci USA 76:615–619
Vola C, Jarry B, Rosset R (1977) Linkage of 5S RNA and 16S+23S RNA genes on the E. coli. chromosome. Mol Gen Genet 153:337–341
Walseth TF, Johnson RA (1979) The enzymatic preparation of [gamma-32P] nucleoside triphosphate, cyclic [32P] AMP, and cyclic [32P] GMP. Biochim Biophys Acta 562:11–31
Woese CR, Maniloff J, Zablen LB (1980) Phylogenetic analysis of the mycoplasmas. Proc Natl Acad Sci USA 77:494–498
Yu PL, John B, Falk H, Drews G (1982) Molecular cloning of the ribosomal RNA genes of the photosynthetic bacterium Rhodopseudomonas capsulata. Mol Gen Genet 188:392–398
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Communicated by A. Böck
Present address: Division of Hematology and Immunology of Internal Medicine, Kanazawa Medical University, Uchinada-Cho, Kahoku-Gun Ishikawa Pref. 920-02, Japan
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Sawada, M., Muto, A., Iwami, M. et al. Organization of ribosomal RNA genes in Mycoplasma capricolum . Mol Gen Genet 196, 311–316 (1984). https://doi.org/10.1007/BF00328064
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DOI: https://doi.org/10.1007/BF00328064