Abstract
Fusarium oxysporum f. sp. dianthi (FOD), incitant of vascular wilt, is the most important pathogen on carnation, where it causes severe losses in all growing areas. The use of resistant carnation cultivars still provides the most effective solution to this problem and indexing is routinely adopted for the obtainment of pathogen-free propagative material, (Baayen and Kleijn, 1989; Garibaldi and Gullino, 1987). Eight physiological races or pathotypes of FOD have been reported (Garibaldi, 1975), and knowledge of the distribution of pathotypes is a basic prerequisite for choosing of suitable resistant varieties in each growing area.
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References
Aloi, C. and Baayen, R.P. (1993) Examination of the relationships between vegetative compatibility groups and races in Fusarium oxysporum f.sp. dianthi, Plant Pathology 42, 839–850.
Assigbetse, K.B., Fernandez, D., Dubois, M.P. and Geiger, J.-P. (1994) Differentiation of Fusarium oxysporum f.sp. vasinfectum races on cotton by random amplified polymorphic DNA (RAPD) analysis, Phytopathology 84, 622–626.
Baayen, R.P. and Kleijn, J. (1989) The Elegans fusaria causing wilt disease of carnation. II. Distinction of vegetative compatibility groups, Netherlands Journal of Plant Pathology 95, 185–194.
Bentley, S., Pegg, K.G. and Dale, J.L. (1994) Optimization of RAPD-PCR fingerprinting to analyse genetic variation within populations of Fusarium oxysporum f.sp. cubense, Journal of Phytopathology 142, 64–78
Ben-Yephet, Y., Reuven, M., Zveibil, A. and Shtienberg, D. (1996) Effects of abiotic variables on the response of carnation cultivars to Fusarium oxysporum f.sp. dianthi, Plant Pathology 45, 98–105.
Fisher, N.L., Burgess, L.W., Toussoun, T.A. and Nelson, P.E. (1982) Carnation leaves as a substrate and for preserving cultures of Fusarium species, Phytopathology 72, 151–153.
Garibaldi, A. (1975) Race differentiation in Fusarium oxysporum f.sp. dianthi (Prill, et Del.) Snyd. et Hans., Mededelingen van de Fakulteit der Landbouwwetenschappen, Rijksuniversiteit Gent 40, 531–537.
Garibaldi, A., Gullino, M.L. (1987) Fusarium wilt of carnation: present situation, problems and perspectives, Acta Horticulturae 216, 45–54.
Grajal-Martin, M.J., Simon, C.J. and Muehlbauer, F.J. (1993) Use of random amplified polymorphic DNA (RAPD) to characterize race 2 of Fusarium oxysporum f.sp. pisi, Phytopathology 83, 612–614.
Katan, T., Hadar, E. and Katan, J. (1989) Vegetative compatibility of Fusarium oxysporum f.sp. dianthi from carnation in Israel, Plant Pathology 38, 376–381.
Manicom, B.Q. and Baayen, R.P. (1993) Restriction fragment length polymorphisms in Fusarium oxysporum f.sp. dianthi and other fusaria from Dianthus species, Plant Pathology 42, 851–857.
Manulis, S., Kogan, N., Reuven, M. and Ben-Yephet, Y. (1994) Use of RAPD technique for identification of Fusarium oxysporum f.sp. dianthi from carnation, Phytopathology 84, 98–101.
Migheli, Q., Berio, T., Gullino, M.L. and Garibaldi, A. (1995) Electrophoretic karyotype variation among pathotypes of Fusarium oxysporum f.sp. dianthi, Plant Pathology 44, 308–315.
Migheli, Q. and Cavallarin, L. (1994) Characterization of antagonistic and pathogenic Fusarium oxysporum isolates by random amplification of polymorphic DNA, Molecular Biotechnology 2, 197–200.
Waalwijk, C., de Koning, J., Baayen, R. and van Pelt, H. (1996) Molecular analysis for virulence in Fusarium oxysporum f.sp. dianthi, Proc. 3rd European Conference on Fungal Genetics, March 27–30, Muenster, Germany, p.209 (abstract).
Welsh, J. and McClelland, M. (1990) Fingerprinting genomes using PCR with arbitrary primers, Nucleic Acids Research 18, 7213–7218.
Williams, J.G.K., Kubelik, A.R., Livak, K.L., Rafalski, J.A. and Tingey, S.V. (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers, Nucleic Acids Research 18: 6531–6535.
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© 1997 Kluwer Academic Publishers
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Migheli, Q., Briatore, E., Andrina, M., Garibaldi, A. (1997). Physiological Race Determination in Fusarium Oxysporum F.SP. Dianthi by RAPD-PCR. In: Dehne, HW., Adam, G., Diekmann, M., Frahm, J., Mauler-Machnik, A., van Halteren, P. (eds) Diagnosis and Identification of Plant Pathogens. Developments in Plant Pathology, vol 11. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0043-1_43
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DOI: https://doi.org/10.1007/978-94-009-0043-1_43
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