Abstract
During the past decade solutions of an increasing variety of problems in the field of molecular genetics have rested on the availability of synthetic polynucleotides. Thus, to cite only a few examples, the elucidation of the genetic code was based on synthesis of the 64 possible trinucleoside diphosphates and on the preparation of poly-nucleotides containing repeating sequences (186, 249). More recently the development of synthetic procedures has culminated in the total synthesis of two tRNA-genes (2, 188, 190). A further useful application has been demonstrated in the use of synthetic oligomers of specific base sequence as specific primers for DNA sequencing (247, 366, 379, 467).Because of the many problems which remain with respect to our understanding in gene function or to future gene manipulation, it seems not surprising that the effort for finding new methods or for improving earlier methods in polynucleotide synthesis still continues or even increases in many laboratories all over the world.
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Kössel, H., Seliger, H. (1975). Recent Advances in Polynucleotide Synthesis. In: Zechmeister, L., Herz, W., Grisebach, H., Kirby, G.W. (eds) Fortschritte der Chemie Organischer Naturstoffe / Progress in the Chemistry of Organic Natural Products. Fortschritte der Chemie Organischer Naturstoffe / Progress in the Chemistry of Organic Natural Products, vol 32. Springer, Vienna. https://doi.org/10.1007/978-3-7091-7083-0_6
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