Abstract
The localization of serotoninergic 5-HT1A receptor binding sites has been extensively studied in the rat central nervous system (CNS) by autoradiography using radioactive ligands such as [3H]8-OH-DPAT (Pazos and Palacios, 1985; Vergé et al., 1986). 5-HT1A binding sites are mainly found in limbic areas (hippocampus, septum, amygdala) which are concerned with the control of mood. Interestingly, some selective agonists (ipsapirone, buspirone) exhibit marked antidepressant and anxiolytic properties (Traber et Glaser, 1987). The raphe nuclei, where are concentrated the somas of 5-HT neurons, are also rich in 5-HT1A binding sites.
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References
Albert PR, Zhou QY, Van Tol HHM, Bunzow JR, Civelli O (1990) Cloning, mRNA tissue distribution, and functional expression of the rat 5-HT1A a receptor gene. J Biol Chem 265: 5825–5832
Correa FMA, Guilhaume SG, Saavedra JM (1988) Quantitative radioimmuno- histochemical method using [125I]-protein A to measure the content of methionine enkephalin in discrete rat brain areas. J Histochem Cytochem 36: 1379–1386
Daval G, Vergé D, Becerril A, Gozlan H, Spampinato U, Hamon M. (1987) Transient expression of 5-HT1A receptor binding sites in some aireas of the rat CNS during postnatal development. Int J Devi Neurosci 5: 171–180
El Mestikawy S, Riad M, Laporte AM, Vergé D, Daval G, Gozlan H, Hamon M (1990) Production of specific anti-rat 5-HT1A a receptor antibodies in rabbits injected with a synthetic peptide. Neurosci Lett 18: 189–192
Gozlan H, Ponchant M, Daval G, Vergé D, Ménard F, Vanhove A, Beaucourt JP, Hamon, M. (1988) [125I]Bolton-Hunter-8-methoxy-2(N-propyl-N-propylamino)- tetralin as a new selective radioligand of 5-HT1A sites in the rat brain. In vitro binding and autoradiographic studies. J Pharmacol Exp Ther 244: 751–759
Pazos A, Palacios JM (1985) Quantitative autoradiographic mapping of serotonin receptors in the rat brain. I. Serotonin-1 receptors. Brain Res 346: 205–230
Sprouse JS, Aghajanian GK (1987) Electrophysiological responses of serotonergic dorsal raphé neurons to 5-HT1A and 5-HT1B d agonists. Synapse 1: 3–9
Traber J, Glaser T (1987) 5-HT1A receptor-relaxed anxiolytics. Trends Pharmacol Sci 8: 432–437
Vergé D, Daval G, Marcinkiewicz M, El Mestikawy S, Gozlan H, Hamon M (1986) Quantitative autoradiography of multiple 5-HT1A receptor subtypes in the brain of control or 5,7- dihydroxytryptamine-treated rats. J Neurosci 6: 3474–3482
Vergé D, Matthiessen L, Bailly Y, Daval G, Kia K, Gozlan H., Hamon M (1990)Localization of 5-HT1A serotonin binding sites in the cerebellum of young rats. Neurochem. Int. in press
Yocca, FD, Iben, I, Meller, E (1990) Lack of receptor reserve (RR) at postsynaptic 5- HT1A receptors linked to adenylate cyclase (AC) in rat hippocampus (HIP). Soc Neurosci 16: 1034 (Abst. 427.4)
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© 1991 Springer-Verlag Berlin Heidelberg
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El Mestikawy, S. et al. (1991). Visualization and Quantification of 5-HT1A Receptors in the Rat Brain by Radioimmunohistochemistry. In: Calas, A., Eugène, D. (eds) Neurocytochemical Methods. NATO ASI Series, vol 58. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-84298-6_25
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DOI: https://doi.org/10.1007/978-3-642-84298-6_25
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