Abstract
Flow cytometric platelet analysis is becoming increasingly popular for both experimental and clinical applications. Flow cytometry (FCM) can feasibly analyze platelet biology both in vivo and ex vivo, providing information on platelet turnover and count, structure, antigen expression, activation state, interaction with other blood components, and response to agonists.
Reduced sample volume, minimal manipulation, and single-shot multiparametric characterization of platelet populations are the main advantages of FCM platelet studies. However, some aspects need to be carefully considered, including activation-dependent changes resulting from inaccurate sample collection, the need for a dedicated operator, and standardization.
Relevant clinical applications include assessment of thrombotic risk in cardiovascular diseases and cancer, monitoring of pharmacological anti-aggregation, and diagnosis of inherited and acquired platelet function disorders. This chapter focuses on the main applications of FCM in studies that are relevant for both research and clinical settings.
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We are grateful to Dr. Giovanni Panico for graphical support.
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Carubbi, C., Masselli, E., Vitale, M. (2017). Flow Cytometry. In: Gresele, P., Kleiman, N., Lopez, J., Page, C. (eds) Platelets in Thrombotic and Non-Thrombotic Disorders. Springer, Cham. https://doi.org/10.1007/978-3-319-47462-5_40
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