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Biotechnology in the Diagnosis and Management of Infectious Diseases

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Current Trends in Plant Disease Diagnostics and Management Practices

Part of the book series: Fungal Biology ((FUNGBIO))

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Abstract

World’s population is continuing to rise, the major target of modern agriculture has to a sustainable increase in yield that keeps pace with the increasing number of mouths to feed. This may be achieved by several approaches, not least by a significant reduction in pre and post harvest losses due to disease. In biotechnology different techniques such as proteomics, transcriptomics and metabolic are now proved to be useful in diagnosis of diseases. Biotechnological procedures can also be used to determine the type and sources of host resistance. In Biotechnology the multiplication and manipulation of genes in any living organisms occurs through novel techniques such as genetic engineering in order to produce new organisms or products that can be used in variety of ways. Conventional plant breeding methods used to developed resistant cultivars for various diseases but it is time consuming process while breeding through the markers i.e. marker assisted breeding may greatly increase the efficiency and effectiveness in plant breeding compared to conventional breeding. The identification of diseases in plant is required to design effective management strategies. Form the last few year Biotechnology offers radical alternatives to detect and enumerate the different diseases. The purpose of this chapter is to provide general information for the diseases diagnosis and its management of infectious diseases thorough biotechnology.

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References

  • Altschul SF, Madden TL, Schaffer AA, Zhang JH, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Audy P, Laroche A, Saindon G, Huang HC, Gilbertson RL (1994) Detection of bean common blight bacteria Xanthomonas compestris pv. Phaseoli and X.C. Phaseoli var. fuscans using the polymerase chain reaction. Phytopathology 84:1185–1192

    Article  CAS  Google Scholar 

  • Bahnweg G, Schulze S, Moller EM, Rosenbrock H, Langebartels C, Sandermann H (1998) DNA isolation from recalsistant materials such as tree roots, bark and forest soil for the detection of fungal pathogens by polymerase chain reaction. Anal Biochem 262:79–82

    Article  CAS  PubMed  Google Scholar 

  • Barnes CW, Szabo LJ (2007) Detection and identification of four common rust pathogens of cereals and grasses using real-time polymerase chain reaction. Phytopathology 97(6):717–727

    Article  CAS  PubMed  Google Scholar 

  • Botstein B, White RL, Skolnick M, Davis RW (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphism. Am J Hum Genet 32:314–331

    CAS  PubMed  PubMed Central  Google Scholar 

  • Brandfass C, Karlovsky P (2006) Simultaneous detection of Fusarium culmorum and Fgraminearum in plant material by duplex PCR with melting curve analysis. BMC Microbiol 6:4–6

    Article  PubMed  PubMed Central  Google Scholar 

  • Chen RS, Chu C, Cheng CW, Chen WY, Tsay JG (2008) Differentiation of two powdery mildews of sunflower (Helianthus annuus) by a PCR-mediated method based on ITS sequences. Eur J Plant Pathol 121(1):1–8

    Article  CAS  Google Scholar 

  • Chi MH, Park S-Y, Lee YH (2009) A quick and safe method for fungal DNA extraction. Plant Pathol J 25(1):108–111

    Article  CAS  Google Scholar 

  • Chunwongse J, Doganlar S, Crossman C, Jiang J, Tanksley SD (1997) High resolution genetic map of Lv resistance locus in tomato. Theor Appl Genet 95:220–223

    Article  CAS  Google Scholar 

  • Dyer PS, Furneaux PA, Douhan G, Murray TD (2001) A multiplex PCR test for determination of mating type applied to the plant pathogens Tapesia yallundae and Tapesia acuformis. Fungal Genet Biol 33(3):173–180

    Article  CAS  PubMed  Google Scholar 

  • Feng J, Hwang R, Chang KF, Hwang SF, Strelkov SE, Gossen BD, Zhou Q (2010) An inexpensive method for extraction of genomic DNA from fungal mycelia. Can J Plant Pathol 32(3):396–401

    Article  CAS  Google Scholar 

  • Fraaije BA, Lovell DJ, Rohel EA, Hollomon DW (1999) Rapid detection and diagnosis of Septoria tritici epidemics in wheat using a polymerase chain reaction PicoGreen assay. J Appl Microbiol 86(4):701–708

    Article  CAS  Google Scholar 

  • González Mendoza D, Argumedo-Delira R, Morales Trejo A, Pulido Herrera A, Cervantes DĂ­az L, Grimaldo-Juarez O, Alarcon A (2010) A rapid method for isolation of total DNA from pathogenic filamentous plant fungi. Genet Mol Res 9(1):162–166

    Article  PubMed  Google Scholar 

  • Graner A, Strengn S, Drescher A, Jin Y, Borovkova I, Steffenson BJ (2000) Molecular mapping of the leaf rust resistance gene Rph7 in barley. Plant Breed 119:389–392

    Article  CAS  Google Scholar 

  • Grund E, Darissa O, Adam G (2010) Application of FTA (R) cards to sample microbial plant pathogens for PCR and RT-PCR. J Phytopathol 158(11–12):750–757

    Article  Google Scholar 

  • Guo JR, Schnieder F, Beyer M, Verreet JA (2005) Rapid detection of Mycosphaerella graminicola in wheat using reverse transcription-PCR assay. J Phytopathol 153(11–12):674–679

    Article  CAS  Google Scholar 

  • Hartung JS, Pruvost UP, Villemont I, Alvarez A (1996) Rapid and sensitive colorimetric detection of Xanthomonas axonopodis pv. Citri by immunocapture and nested polymerase chain reaction assay. Phytopathology 86:95–101

    Article  CAS  Google Scholar 

  • Helguera IA, Dubcovsky KJ (2000) Development of PCR markers for the wheat leaf rust resistance gene Lr 47. Theor Appl Genet 100:1137–1143

    Article  CAS  Google Scholar 

  • Hong SY, Kang MR, Cho EJ, Kim HK, Yun SH (2010) Specific PCR detection of four quarantine Fusarium Species in Korea. Plant Pathol J 26(4):409–416

    Article  CAS  Google Scholar 

  • Huang Q, Hsam SLK, Zeller FJ, Wenzel G, Mohler V (2000) Molecular mapping of the wheat powdery mildew resistance gene Pm24 and marker validation for molecular breeding. Theor Appl Genet 101:407–414

    Article  CAS  Google Scholar 

  • Hyakumachi M, Priyatmojo A, Kubota M, Fukui H (2005) New anastomosis groups, AG-T and AG-U, of binucleate Rhizoctonia spp. causing root and stem rot of cutflower and miniature roses. Phytopathology 95(7):784–792

    Article  PubMed  Google Scholar 

  • Jana T, Sharma TR, Singh NK (2005) SSR-based detection of genetic variability in the charcoal root rot pathogen Macrophomina phaseolina. Mycol Res 109:81–86

    Article  CAS  PubMed  Google Scholar 

  • Jeeva ML, Mishra AK, Vidyadharan P, Misra RS, Hegde V (2010) A species-specific polymerase chain reaction assay for rapid and sensitive detection of Sclerotium rolfsii. Australas Plant Pathol 39(6):517–523

    Article  Google Scholar 

  • Joshi SP, Prabhakar K, Ranjekar PK, Gupta VS (2011) Molecular markers in plant genome analysis. 15:1–19. http://www.ias.ac.in/currsci/jul25/articles

  • Kim YT, Cho M, Jeong JY, Lee HB, Kim SB (2010) Application of terminal restriction fragment length polymorphism (T-RFLP) analysis to monitor effect of biocontrol agents on rhizosphere microbial community of hot pepper (Capsicum annuum L.). J Microbiol 48(5):566–572

    Article  CAS  PubMed  Google Scholar 

  • Langrell SRH, Glen M, Alfenas AC (2008) Molecular diagnosis of Puccinia psidii (guava rust) – a quarantine threat to Australian eucalypt and Myrtaceae biodiversity. Plant Pathol 57(4):687–701

    Article  CAS  Google Scholar 

  • Levesue CA, Vrain TC, De Boer SH (1994) Development of species specific probe for phythium ultimum using amplified ribosomal DNA. Phytopathology 84:474–478

    Article  Google Scholar 

  • MartĂ­nez GarcĂ­a LB, Armas C, Miranda JD, Padilla FM, Pugnaire FI (2011) Shrubs influence arbuscular mycorrhizal fungi communities in a semi-arid environment. Soil Biol Biochem 43(3):682–689

    Article  Google Scholar 

  • Meng J, Wang Y (2010) Rapid detection of Phytophthora nicotianae in infected tobacco tissue and soil samples based on its Ypt1 gene. J Phytopathol 158(1):1–7

    Article  CAS  Google Scholar 

  • Mohan M, Nair S, Bhagwat A, Krishna TG, Yano M, Bhatia CR, Sasaki T (1997) Genome mapping, molecular markers and marker assisted selection in crop plants. Mol Breed 3:87–103

    Article  CAS  Google Scholar 

  • Moradi M, Oerke EC, Steiner U, Tesfaye D, Schellander K, Dehne HW (2010) Microbiological and Sybr Green real-time PCR detection of major Fusarium head blight pathogens on wheat ears. Microbiology 79(5):646–654

    Article  CAS  Google Scholar 

  • Mullis KB, Faloona FA (1987) Specific synthesis of DNA in vitro via a polymerasecatalyzed chain-reaction. Methods Enzymol 155:335–350

    Article  CAS  PubMed  Google Scholar 

  • Niu C, Kebede H, Auld DL, Woodward JE, Burow G, Wright RJ (2008) A safe inexpensive method to isolate high quality plant and fungal DNA in an open laboratory environment. Afr J Biotechnol 7(16):2818–2822

    CAS  Google Scholar 

  • Okubara PA, Schroeder KL, Paulitz TC (2008) Identification and quantification of Rhizoctonia solani and R. oryzae using real-time polymerase chain reaction. Phytopathology 98(7):837–847

    Article  CAS  PubMed  Google Scholar 

  • Powell W, Machary GC, Provan J (1996) Polymorphism revealed by simple sequence repeats. Trends Plant Sci 1:215–222

    Article  Google Scholar 

  • Procunier JD, Knox RE, Bernier AN, Grag MA, Howes NK (1997) DNA markers linked to a T10 loose smut resistance gene in wheat (Triticum aestivum L.). Genome 40:176–179

    Article  CAS  PubMed  Google Scholar 

  • Qin L, Fu Y, Xie J, Cheng J, Jiang D, Li G, Huang J (2011) A nested-PCR method for rapid detection of Sclerotinia sclerotiorum on petals of oilseed rape (Brassica napus). Plant Pathol 60(2):271–277

    Article  CAS  Google Scholar 

  • Sato M, Watanabe K, Yazawa M, Takikawa Y, Nishiyama K (1997) Detection of new ethylene producing bacteria, Pseudomonas syringae pvs. Cannabina and sami, by PCR amplification of gene for the ethylene forming enzyme. Phytopathology 87:1192–1196

    Article  CAS  PubMed  Google Scholar 

  • Schmidt H, Taniwaki MH, Vogel RF, Niessen L (2004) Utilization of AFLP markers for PCR-based identification of Aspergillus carbonarius and indication of its presence in green coffee samples. J Appl Microbiol 97(5):899–909

    Article  CAS  PubMed  Google Scholar 

  • Schroeder KL, Okubara PA, Tambong JT, Levesque CA, Paulitz TC (2006) Identification and quantification of pathogenic Pythium spp. from soils in eastern Washington using real-time polymerase chain reaction. Phytopathology 96(6):637–647

    Article  CAS  PubMed  Google Scholar 

  • Sharma TR, Prachi, Singh BM (1999) Application of polymerase chain reaction in phytopathogenic microbes. Indian J Microbiol 39:79–91

    Google Scholar 

  • Sharma TR, Tewari JP, Singh BM (2002) Use of polymerase chain reaction of the synthesis of non radioactive DNA probes in phytopathogenic ungi. In: Sekhawat GS et al (eds) Molecular approaches for plant disease management. Malhotra Publishing House, New Delhi, p 350

    Google Scholar 

  • Sheridan GEC, Masters CI, Shallcross JA, Mackey BM (1998) Detection of mRNA by reverse transcription-PCR as an indicator of viability in Escherichia coli cells. Appl Environ Microbiol 64(4):1313–1318

    CAS  PubMed  PubMed Central  Google Scholar 

  • Singh Y (2009) Non radioactive gene probes. AGROBIOS News Lett 08(02):07–10

    Google Scholar 

  • Singh M, Singh RP (1997) Potato virus Y detection: sensitivity of RT-PCR depends on the size of fragment amplified. Can J Plant Pathol Rev Can Phytopathol 19:149–155

    Article  CAS  Google Scholar 

  • Stewart JE, Kim MS, James RL, Dumroese RK, Klopfenstein NB (2006) Molecular characterization of Fusarium oxysporum and Fusarium commune isolates from a conifer nursery. Phytophathology 96:1124–1133

    Article  CAS  Google Scholar 

  • Stringari D, Glienke C, de Christo D, Maccheroni W, de Azevedo JL (2009) High molecular diversity of the fungus Guignardia citricarpa and Guignardia mangiferae and new primers for the diagnosis of the Citrus Black Spot. Braz Arch Biol Technol 52(5):1063–1073

    Article  CAS  Google Scholar 

  • Suzuki S, Taketani H, Kusumoto KI, Kashiwagi Y (2006) High-throughput genotyping of filamentous fungus Aspergillus oryzae based on colony direct polymerase chain reaction. J Biosci Bioeng 102(6):572–574

    Article  CAS  PubMed  Google Scholar 

  • Thies JE (2007) Soil microbial community analysis using terminal restriction fragment length polymorphisms. Soil Sci Soc Am J 71(2):579–591

    Article  CAS  Google Scholar 

  • Torres-Calzada C, Tapia-Tussell R, Quijano-Ramayo A, Martin-Mex R, Rojas-Herrera R, Higuera-Ciapara I, Perez-Brito D (2011) A species-specific polymerase chain reaction assay for rapid and sensitive detection of Colletotrichum capsici. Mol Biotechnol 49(1):48–55

    Article  CAS  PubMed  Google Scholar 

  • VanderLee T, DeWitte I, Drenth A, Alfonso C, Govers F (1997) AFLP linkage map of the oomycete Phytophthora infestans. Fungal Genet Biol 21(3):278–29

    Article  CAS  Google Scholar 

  • Vos P, Hogers R, Bleeker M, Reijans M, Van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabean M (1995) AFLP- A new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1991) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531–6535

    Article  Google Scholar 

  • Winton LM, Krohn AL, Leiner RH (2007) Microsatellite markers for Sclerotinia subarctica nom. prov., a new vegetable pathogen of the High North. Mol Ecol Notes 7(6):1077–1079, ISSN 1471-8278

    Article  CAS  Google Scholar 

  • Wu CP, Chen GY, Li B, Su H, An YL, Zhen SZ, Ye JR (2011) Rapid and accurate detection of Ceratocystis fagacearum from stained wood and soil by nested and realtime PCR. For Pathol 41(1):15–21

    Google Scholar 

  • Yang F, Jensen JD, Svensson B, Jorgensen HJL, Collinge DB, Finnie C (2010) Analysis of early events in the interaction between Fusarium graminearum and the susceptible barley (Hordeum vulgare) cultivar Scarlett. Proteomics 10(21):3748–3755

    Article  CAS  PubMed  Google Scholar 

  • Zaccaro RP, Carareto-Alves LM, Travensolo RF, Wickert E, Lemos EGM (2007) Use of molecular marker SCAR in the identification of Fusarium subglutinans, causal agent of mango malformation. Rev Bras Frutic 29(3):563–570

    Article  Google Scholar 

  • Zelaya Molina LX, Ortega MA, Dorrance AE (2011) Easy and efficient protocol for oomycete DNA extraction suitable for population genetic analysis. Biotechnol Lett 33(4):715–720

    Article  CAS  PubMed  Google Scholar 

  • Zhang Z, Zhu Z, Ma Z, Li H (2009) A molecular mechanism of azoxystrobin resistance in Penicillium digitatum UV mutants and a PCR-based assay for detection of azoxystrobin-resistant strains in packing- or store-house isolates. Int J Food Microbiol 131:157–161

    Article  CAS  PubMed  Google Scholar 

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Correspondence to Amit Kumar .

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Kumar, A., Sengar, R.S., Singh, R., Rani, A., Girdharwal, V., Shukla, G. (2016). Biotechnology in the Diagnosis and Management of Infectious Diseases. In: Kumar, P., Gupta, V., Tiwari, A., Kamle, M. (eds) Current Trends in Plant Disease Diagnostics and Management Practices. Fungal Biology. Springer, Cham. https://doi.org/10.1007/978-3-319-27312-9_15

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