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Systemic Approaches to Resolve Spatiotemporal Regulation of GTPase Signaling

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GTPases

Part of the book series: SpringerBriefs in Plant Science ((BRIEFSPLANT))

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Abstract

Rho-related GTPases are the signaling switches that pair extracellular changes to intracellular signaling cascades. Their proficiency in the regulation of cellular signaling and modifications requires rigorous control of their spatial and temporal activities. The observation of spatiotemporal activity of Rho proteins cannot be achieved only through biochemical assays. The advent of fluorescent probes and optical reporters has transformed the precise detection and measurement of cellular activity in real time. Conventionally, the presumed cellular activity of a GTPase protein could be assumed by the manifestation of the cytoskeletal and cellular adhesion. Even though these methods provided information regarding relative activity of Rho GTPases, no information could be retrieved about their spatiotemporal dynamics. This necessitates the development of new tools to enable quantitative and spatial detection of the activity of these proteins. Some of the recent studies have devised new techniques to provide an insight into understanding the regulation of protein activities in living cells.

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Authors and Affiliations

  1. Department of Plant Molecular Biology, Delhi University South Campus, Dhaula Kuan, New Delhi, India

    Girdhar K. Pandey, Manisha Sharma & Amita Pandey

  2. Division of Biosciences and Bioinformatics, Myongji University, Kyunggi-do, Republic of South Korea

    Thiruvenkadam Shanmugam

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  1. Girdhar K. Pandey
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  2. Manisha Sharma
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  4. Thiruvenkadam Shanmugam
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Pandey, G.K., Sharma, M., Pandey, A., Shanmugam, T. (2015). Systemic Approaches to Resolve Spatiotemporal Regulation of GTPase Signaling. In: GTPases. SpringerBriefs in Plant Science. Springer, Cham. https://doi.org/10.1007/978-3-319-11611-2_9

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