Abstract
Two methods that use chemoselective ligation chemistry to prepare peptide and smallmolecule microarrays are described here. The first method involves the functionalization of a glass slide with a glyoxylyl group, followed by chemoselective ligation of small molecules or peptides to the functionalized surface via a covalent bond. In the second method, peptides or small molecules are first conjugated to a macromolecular scaffold. The final ligand-scaffold conjugates are then spotted and adsorbed onto the solid surface. Three different assay methods to screen such chemical microarrays are described.
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Acknowledgments
The authors would like to thank Amanda Enstrom for the assistance with the manuscript. This work was supported by NSF Grant MCB9728399, and NIH Grants R33CA-86364, R33CA-89706, and R01CA-098116.
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Marik, J., Lam, K.S. (2005). Peptide and Small-Molecule Microarrays. In: Zanders, E.D. (eds) Chemical Genomics. Methods in Molecular Biology™, vol 310. Humana Press. https://doi.org/10.1007/978-1-59259-948-6_15
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DOI: https://doi.org/10.1007/978-1-59259-948-6_15
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