Abstract
Protein microarrays have many potential applications in high-throughput analysis of protein function. However, simple, reproducible, and robust methods for array fabrication are required. Here we discuss the background to different routes to array fabrication and describe in detail one approach in which the purification and immobilization procedures are combined into a single step, dramatically simplifying the array fabrication process. We illustrate this approach by reference to the creation of an array of p53 variants, and discuss methods for assay and data analysis on such arrays.
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Acknowledgments
The authors thank Drs. Joe Boutell, Ben Godber, and Mike Dyson for their help in generating the data and the procedures detailed in this chapter, and Dr. Roland Kozlowski for helpful discussion along the way. This work was supported by Procognia Ltd.
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Blackburn, J.M., Hart, D.J. (2005). Fabrication of Protein Function Microarrays for Systems-Oriented Proteomic Analysis. In: Zanders, E.D. (eds) Chemical Genomics. Methods in Molecular Biology™, vol 310. Humana Press. https://doi.org/10.1007/978-1-59259-948-6_14
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DOI: https://doi.org/10.1007/978-1-59259-948-6_14
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