Abstract
Recombinant adenoviruses are versatile tools for gene delivery into mammalian tissue-culture cells. Adenoviruses can infect both dividing and nondividing cells, with efficiencies approaching 100%. Here we describe the use of adenoviruses to express reporter proteins in high-throughput cell-based assays.
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Acknowledgments
The development of the methods described in this chapter were supported by grants from the NCI (CA78048) and NIGMS (GM66492), and by support from Merck & Co. and Merck KGaA.
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Hoyt, J., King, R.W. (2005). Adenoviral Expression of Reporter Proteins for High-Throughput Cell-Based Screening. In: Zanders, E.D. (eds) Chemical Genomics. Methods in Molecular Biology™, vol 310. Humana Press. https://doi.org/10.1007/978-1-59259-948-6_13
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DOI: https://doi.org/10.1007/978-1-59259-948-6_13
Publisher Name: Humana Press
Print ISBN: 978-1-58829-399-2
Online ISBN: 978-1-59259-948-6
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