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Preparative liquid chromatography (PLC) potentially can be undertaken using all of the techniques described in Chapter 11, and the basic requirement for efficient preparation is to obtain the maximum throughput in unit time. This requires optimization of the methods employed, which is the subject of this chapter. However, there is an additional constraint in preparative protein separations; that is, the prepared proteins should retain their biological activity, and this imposes severe restraints upon the operating system. Three effects can be listed that potentially can affect protein denaturization: (1) the “hardness” of the surface, (2) temperature effects, and (3) shear forces.
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© 1988 The Humana Press Inc
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Simpson, C. (1988). Preparative Liquid Chromatography. In: Franks, F. (eds) Characterization of Proteins. Biological Methods. Humana Press. https://doi.org/10.1007/978-1-59259-437-5_13
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DOI: https://doi.org/10.1007/978-1-59259-437-5_13
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