Abstract
The application of liquid chromatography (LC) to protein separations has advanced significantly over the last few years, and three of the conventional LC techniques have proven to be valuable in this respect. All LC methodology may be applied to the separation of proteins to a greater or lesser extent; namely, liquid/solid (adsorption) (LSC), liquid/liquid (partition) (LLC), bonded phase (BPC), particularly reversed phase (RPC), hydrophobic interaction (HIC) (effectively a modified form of reversed-phase chromatography), ion exchange (IEC), and size exclusion [SEC (GPC)], together with the “newer” techniques, affinity chromatography (AC), chromatofocusing (CF), and field flow fractionation (FFF). Of the methods mentioned above, four [BPC (RX), HIC, IEC, and SEC] have proven to be the most useful, possibly because they are simple extensions of known methodologies applied to proteins, but also because they are the “softer” of the techniques, less liable to bring about denaturation of the protein, or easier to control. However, care has to be exercised, particularly with BPC (RX) and HIC, because the nonpolar nature of the stationary phase can have a deleterious effect on proteins. AC has had many triumphs, and it is capable of highly selective separations, but is a difficult technique to utilize in that the construction of the bound substrate to the matrix is often chemically demanding
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© 1988 Humana Press Inc.
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Simpson, C. (1988). Analytical Liquid Chromatography of Proteins. In: Franks, F. (eds) Characterization of Proteins. Biological Methods. Humana Press. https://doi.org/10.1007/978-1-59259-437-5_11
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DOI: https://doi.org/10.1007/978-1-59259-437-5_11
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