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Analysis of Amino Acids and Small Peptides

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Characterization of Proteins

Part of the book series: Biological Methods ((BM))

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Abstract

Proteins are composed of amino acids, and the nature and composition of the various constituent amino acids confer upon the protein its characteristic properties. Of even greater importance is the order in which the constituent amino acids are arranged in the protein molecule and the tertiary structure the protein molecule may adopt, although we may at this stage be unable to relate structure to protein function. Thus there are two different analyses to be performed: (1) the proportions of the various naturally occurring ammo acids that are present in a given protein, and (2) the determination of the order in which the constituent amino acids are arranged in the protein molecule. Alternatively, the straight amino acid composition of proteinaceous material can provide information as to the source of a particular food for human consumption or animal feed, or indicate the nature of a protein from the constituent amino acids present, e.g., whether the protein is fibrous or globular in character. Thus amino acid analysis is an important tool in the biochemists armory, indeed one that is undertaken routinely on a day-to-day basis.

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References

  1. R. Consdon, A. H. Gordon and A. J. P. Martin, Biochem. J. 38, 224 (1944).

    Google Scholar 

  2. C. E. Dent, Biochem. J. 43, 169 (1948).

    CAS  Google Scholar 

  3. G. L. Kowkabany and H. G. Cassidy, Anal. Chem. 22, 817 (1950).

    Article  CAS  Google Scholar 

  4. E. F. McFarren, Anal. Chem. 23, 168 (1951).

    Article  CAS  Google Scholar 

  5. L. Fowden, Biochem. J. 50, 355 (1952).

    PubMed  CAS  Google Scholar 

  6. C. S. Hanes, Can. J. Biochem. Physiol. 39, 119 (1961).

    Article  PubMed  CAS  Google Scholar 

  7. E. H. M. Wade, A. T. Matheson, and C. S. Hanes, Can. J. Biochem. Physiol. 39, 417 (1961).

    Article  Google Scholar 

  8. T. B Moore and C. G. Baker, J. Chromatog. 1, 513 (1958).

    Article  Google Scholar 

  9. D. Gross, Nature 184, 1298 (1955).

    Article  Google Scholar 

  10. I. Smith, ed., Chromatographic and Electrophoretic Techniques vol. 2, 4th Ed. Heinemann, London (1976).

    Google Scholar 

  11. M. Brenner and A. Niederweuser, Experimentia 16, 373 (1960).

    Article  Google Scholar 

  12. A. R. Fahmy, A. Niederwieser, G. Pataki, and M. Brenner, Hely. Chim. Acta 44, 2193 (1961).

    Article  Google Scholar 

  13. N. Grinberg and S. Weinstein, J. Chromatog. 303, 251 (1984).

    Article  CAS  Google Scholar 

  14. I. R. Hunter, K. P. Dimik, and J. Corse, Chem. Ind. 294, (1956).

    Google Scholar 

  15. S. L. MacKenzie and D. Tenaschuck, J. Chromatog. 97, 19 (1974).

    Article  CAS  Google Scholar 

  16. P. Husek, J. Chromatog. 234, 381 (1982).

    Article  CAS  Google Scholar 

  17. P. Edman, Acta Chem. Stand. 4, 277–283, (1950).

    Article  CAS  Google Scholar 

  18. S. Moore, D. H. Spackman, and W. H. Stein, Anal. Chem. 30, 1185 (1958).

    Article  CAS  Google Scholar 

  19. D. H. Spackman, W. H. Stein, and S. Moore, Anal. Chem. 31, 1190 (1958).

    Article  Google Scholar 

  20. P. B. Hamilton, D. Bogue, and R. A. Anderson, Anal. Chem. 32,1782 (1960).

    Article  CAS  Google Scholar 

  21. K. A. Piez and L. Morris, Analyt. Biochem. 1, 187 (1960).

    Article  PubMed  CAS  Google Scholar 

  22. S. Udenfriend, S. Stein, P. Bohlen, W. Dairman, W. Leimgruber, and M. Wiegele, Science 178, (1972).

    Google Scholar 

  23. J. R. Benson and P. E. Hare, Proc. NatI. Acad. Sci. USA 72, 619 (1975).

    Article  CAS  Google Scholar 

  24. C. Murren, D. Stelling, and G. Felstead, J. Chromatog. 115, 236 (1975).

    Article  CAS  Google Scholar 

  25. R. Errser, Lab. Equip. Dig. 17, (6) 61 (1979).

    Google Scholar 

  26. S. J. DiMari, J. P. Robinson, and J. H. Hash, J. Chromatog. 213, 91 (1981).

    Article  CAS  Google Scholar 

  27. R. L. Cunico, R. Simpson, L. Correia, and C T. Wehr, J. Chromatog. 336, 105 (1984).

    Article  CAS  Google Scholar 

  28. W. G. Kruggel and R. V. Lewis Anal. Biochem. (submitted).

    Google Scholar 

  29. S. A. Cohen, T. L. Tarvin, and B. A. Biblingmeyer, Internat. Lab. Oct., 68 (1984).

    Google Scholar 

  30. J-Y. Chang, R. Knecht, and D. G. Braun, Meth. Enzymol. 91,41 (1981).

    Article  Google Scholar 

  31. Y. Tauphi, D. E. Smith, W. Linder, and B. L. Karger, Anal. Biochem. 115, 123 (1981).

    Article  Google Scholar 

  32. V. T. Wiedmeier, S. P. Porterfield, and C. E. Hendrich, J. Chromatog. 231, 410 (1982).

    Article  CAS  Google Scholar 

  33. N. B. Levina and I. V. Nazimov, J. Chromatog. 286, 207 (1984).

    Article  CAS  Google Scholar 

  34. B. Oray, H S. Lu, and R. W. Gracy, J. Chromatog. 270, 253 (1983).

    Article  CAS  Google Scholar 

  35. S. Kobayashi and K. Imai, Anal. Chem. 52, 424 (1980).

    Article  CAS  Google Scholar 

  36. K. Samejima, W. Dairman, and S. Udenfriend, Anal. Biochem. 42, 222 (1971).

    Article  PubMed  CAS  Google Scholar 

  37. M. Weigele, S. L. DeBernardo, J. P. Tengi, and W. Leimgruber, J. Am. Chem. Soc. 94 (16), 5927 (1974).

    Article  Google Scholar 

  38. M. Roth, Anal. Chem. 43, 880 (1971).

    Article  PubMed  CAS  Google Scholar 

  39. P. Lindrith and K, Mopper, Anal. Chem. 51, 1667 (1979).

    Article  Google Scholar 

  40. J. D. H. Cooper, G. Ogden, J, McIntosh, and D C Turnell, Anal. Biochem. 142, 98 (1984).

    Article  PubMed  CAS  Google Scholar 

  41. J. D. H. Cooper, M. T Lewis, and D. C. Turnell, J. Chromatog. 285, 490 (1984)

    Article  CAS  Google Scholar 

  42. L. Johnson, S. Lagerkvlst, P. Lindroth, M. Ahnoff, and K. Martinsson, Anal. Chem. 54, 939 (1982).

    Article  CAS  Google Scholar 

  43. M. Doury-Berthod, C. Poitrenaud, and B. Tremillon, J. Chromatog. 179, 37 (1979).

    Google Scholar 

  44. E. Gil-Av, B. Feibush, and R. Charles-Sigler, in Gas Chromatography A. B. Littlewood, Institute of Petroleum, London (1966). p. 227.

    Google Scholar 

  45. P. E. Hare and E. Gil-Av, Science 204, 1226 (1979).

    Article  PubMed  CAS  Google Scholar 

  46. N. Nimura, A. Toyama, and T. Kinoshita, J. Chromatog. 316, 547 (1984).

    Article  CAS  Google Scholar 

  47. D. W. Armstrong and W. DeMond, J. Chromatogr. Sci. 22, 411 (1984).

    CAS  Google Scholar 

  48. S. Yuasa, M. Itoh, and A. Shimada, J, Chromatogr. Sci. 22, 288 (1984).

    CAS  Google Scholar 

  49. F. M. Everaerts, J. L. Beckers, and Th. P. E. M. Verheggen, Isotuchophoresis J. Chroma. vol. 6, Elsevier, Amsterdam(197e).

    Google Scholar 

  50. S-G. Hjalmarsson and A. Baldesten, Capillary Isotachophoresis CRC (1981).

    Google Scholar 

  51. J. W. Jorgenson and K. D. Lukacs, Anal. Chem. 53, 1298 (1981).

    Article  CAS  Google Scholar 

  52. J. W. Jorgenson and K. D. Lukacs, J. chromatog. 218, 209 (1981).

    Article  CAS  Google Scholar 

  53. A. G. Georgiadis and J. W. Coffey, Anal. Boichem. 56, 121 (1973).

    Article  CAS  Google Scholar 

  54. W. G. Kruggel and R. V. Lewis, AnaI. Biochem., in press.

    Google Scholar 

  55. F. M. Eveaerts, J. L. Beckers, and Th. P. E. M. Verheggen, J. Chromatog. 19, 129 (1976).

    Article  Google Scholar 

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© 1988 Humana Press Inc.

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Simpson, C. (1988). Analysis of Amino Acids and Small Peptides. In: Franks, F. (eds) Characterization of Proteins. Biological Methods. Humana Press. https://doi.org/10.1007/978-1-59259-437-5_10

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  • DOI: https://doi.org/10.1007/978-1-59259-437-5_10

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-109-8

  • Online ISBN: 978-1-59259-437-5

  • eBook Packages: Springer Book Archive

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