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Microspore Embryogenesis Through Anther Culture in Citrus clementina Hort. ex Tan.

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In Vitro Embryogenesis in Higher Plants

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1359))

Abstract

Anther culture is a biotechnological method that allows to obtain, in one step, homozygous plants, very important to plant breeding, due to their numerous applications in mutation research, selection, genome sequencing, genetic analysis, and transformation. To induce the microspores, i.e., the immature male gametes, to switch from the normal gametophytic pathway to the sporophytic one, it is necessary to submit them to a type of stress, such as high or low temperature, starvation, or magnetic field. Stress can be applied to the donor plants and/or the floral buds or the anthers or the isolated microspores, before or during the culture. In this chapter, the protocol to induce gametic embryogenesis from anther culture of several cultivars of Citrus clementina Hort. ex Tan. is reported.

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Author information

Authors and Affiliations

  1. Dipartimento di Scienze degli Alimenti, Università degli Studi di Parma, Parco Area delle Scienze, 48/A, 43124, Parma, Italy

    Benedetta Chiancone

  2. Dipartimento di Scienze Agrarie e Forestali, Università degli Studi di Palermo, viale delle Scienze, 11 Ed. 4, 90128, Palermo, Italy

    Maria Antonietta Germanà

Authors
  1. Benedetta Chiancone
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  2. Maria Antonietta Germanà
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Correspondence to Maria Antonietta Germanà .

Editor information

Editors and Affiliations

  1. Dipartimento DEMETRA, Università degli Studi di Palermo, Palermo, Italy

    Maria Antonietta Germana

  2. National Research Council (CNR) IVALSA/Istituto per la Valorizzazione, Sesto Fiorentino, Florence, Italy

    Maurizio Lambardi

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Chiancone, B., Germanà, M.A. (2016). Microspore Embryogenesis Through Anther Culture in Citrus clementina Hort. ex Tan.. In: Germana, M., Lambardi, M. (eds) In Vitro Embryogenesis in Higher Plants. Methods in Molecular Biology, vol 1359. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3061-6_27

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  • DOI: https://doi.org/10.1007/978-1-4939-3061-6_27

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3060-9

  • Online ISBN: 978-1-4939-3061-6

  • eBook Packages: Springer Protocols

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