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Methods for Assessing the Effects of Galectins on Leukocyte Trafficking

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Galectins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1207))

Abstract

Numerous protocols exist for investigating leukocyte recruitment both in vitro and in vivo. Here we describe three of these methods; an in vitro flow chamber assay, intravital microscopy, and zymosan-induced peritonitis, and give details as to how they can be used to study the actions of galectins on this crucial process.

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References

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Authors and Affiliations

  1. William Harvey Research Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, Charterhouse Square, EC1M 6BQ, London, UK

    Beatrice R. Gittens, Rachael D. Wright & Dianne Cooper

Authors
  1. Beatrice R. Gittens
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  2. Rachael D. Wright
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  3. Dianne Cooper
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Corresponding author

Correspondence to Dianne Cooper .

Editor information

Editors and Affiliations

  1. Center for Transfusion and Cellular Therapies, Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia, USA

    Sean R. Stowell

  2. Department of Biochemistry, Emory University School of Medicine, Atlanta, GA, Georgia, USA

    Richard D. Cummings

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© 2015 Springer Science+Business Media New York

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Gittens, B.R., Wright, R.D., Cooper, D. (2015). Methods for Assessing the Effects of Galectins on Leukocyte Trafficking. In: Stowell, S., Cummings, R. (eds) Galectins. Methods in Molecular Biology, vol 1207. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1396-1_9

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  • DOI: https://doi.org/10.1007/978-1-4939-1396-1_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1395-4

  • Online ISBN: 978-1-4939-1396-1

  • eBook Packages: Springer Protocols

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