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Generation of Induced Pluripotent Stem Cells from Mouse Adipose Tissue

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Mouse Genetics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1194))

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Abstract

The discovery that embryonic stem (ES) cell-like cells can be generated by simply over-expressing four key genes in adult somatic cells has changed the face of regenerative medicine. These induced pluripotent stem (iPS) cells have a wide range of potential uses from drug testing and in vitro disease modeling to personalized cell therapies for patients. However, prior to the realization of their potential, many issues need to be considered. One of these is the low-efficiency formation of iPSC. It has been extensively demonstrated that the somatic cell type can greatly influence reprogramming outcomes. We have shown that adipose tissue-derived cells (ADCs) can be easily isolated from adult animals and can be reprogrammed to a pluripotent state with high efficiency. Here, we describe a protocol for the high-efficiency derivation of ADCs and their subsequent use to generate mouse iPSC using Oct4, Sox2, Klf4, and cMyc retroviral vectors.

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Author information

Authors and Affiliations

  1. Research Department of Haematology, UCL Cancer Institute, University College London, London, WC1E 6BT, UK

    Pollyanna Agnes Goh

  2. Stem Cells and Reprogramming Group, Biological Engineering Laboratories, Faculty of Engineering, Monash University, Clayton, VIC, 3800, Australia

    Paul J. Verma

  3. Turretfield Research Centre, South Australian Research & Development Institute, Rosedale, SA, 5350, Australia

    Paul J. Verma

Authors
  1. Pollyanna Agnes Goh
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  2. Paul J. Verma
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Corresponding author

Correspondence to Paul J. Verma .

Editor information

Editors and Affiliations

  1. Stem Cell Regulation and Animal Aging Section, Basic Research Laboratory,, National Cancer Institute, National Institutes of Health, Frederick, MD, USA

    Shree Ram Singh

  2. Genetically Engineered Mouse Modeling Core/Department of Molecular Virology, Immunology and Medical Genetics, Wexner Medical Center, Comprehensive Cancer Center, The Ohio State University, Columbus, OH, USA

    Vincenzo Coppola

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© 2014 Springer Science+Business Media New York

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Goh, P.A., Verma, P.J. (2014). Generation of Induced Pluripotent Stem Cells from Mouse Adipose Tissue. In: Singh, S., Coppola, V. (eds) Mouse Genetics. Methods in Molecular Biology, vol 1194. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1215-5_14

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  • DOI: https://doi.org/10.1007/978-1-4939-1215-5_14

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1214-8

  • Online ISBN: 978-1-4939-1215-5

  • eBook Packages: Springer Protocols

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